Methods The PCR-based detection technique for five Salmonella species was tested under O.I.E. requirements, to determine specificity, sensitivity, and repeatability. Primers were calculated by OLIGO software and produced with HPLC purification. Amplification was conducted by a T3000 Biometra PCR-machine. Testing was performed on a panel of five basic agents: Salmonella enterica ser. Enteritidis...

Fowl typhoid (FT) and pullorum disease (PD) are septicaemic diseases, primarily of chickens and turkeys, caused by Gram negative bacteria, Salmonella Gallinarum and S. Pullorum, respectively. Clinical signs in chicks and poults include anorexia, diarrhoea, dehydration, weakness and high mortality. In mature fowl, FT and PD are manifested by decreased egg production, fertility, hatchability and ...

Salmonella enterica serovar Pullorum (S. pullorum) causes pullorum disease in poultry and results in great economic losses to the poultry industry. Although an eradication program has been successfully performed in some countries, it remains a major threat to countries with poor poultry disease surveillance. Currently there are no effective control measures for pullorum disease except eradicati...

Salmonella enterica subsp. enterica serovar Gallinarum biovar Pullorum (Salmonella Pullorum) is highly adapted to chickens causing an acute systemic disease that results in high mortality. Vaccination represents one approach for promoting animal health, food safety and reducing environmental persistence in Salmonella control. An important consideration is that Salmonella vaccination in poultry ...

Salmonella enterica serovar Pullorum is a fowl-adapted bacterial pathogen that causes dysentery (pullorum disease). Host adaptation and special pathogenesis make S. enterica serovar Pullorum an exceptionally good system for studies of bacterial evolution and speciation, especially regarding pathogen-host interactions and the acquisition of pathogenicity. We constructed a genome map of S. enteri...

Salmonella gallinarum with the biotypes gallinarum and pullorum causes two septicaemic diseases in poultry: fowl typhoid (FT) and pullorum disease. The former Salmonella pullorum serovar is not recognised anymore as such. Both biotypes are differentiated by a few biochemical and molecular tests. Mammals may be infected without showing any illness. In contrast to zoonotic salmonella serovars, Sa...

Myeloid differentiation primary response gene 88 (MYD88), a universal adapter protein, plays an important role in activating the nuclear factor-κB (NF-κB) and regulating the expression of proinflammatory genes like tumor necrosis factor (TNF) and interleukin-1 (IL-1), which were highly involved in Salmonella Pullorum infection. To detect the relationship between polymorphisms of the MyD88 gene ...

Salmonella enterica serovar Pullorum causes persistent infections in laying hens. Splenic macrophages are the main site of persistence. At sexual maturity, numbers of bacteria increase and spread to the reproductive tract, which may result in vertical transmission to eggs or chicks. In this study we demonstrate that both male and female chickens may develop a carrier state following infection b...