(1) For all real numbers s1, s3, s4, l such that s1 ≤ s3 and s1 ≤ s4 and 0 ≤ l and l ≤ 1 holds s1 ≤ (1− l) · s3 + l · s4. (2) For all real numbers s1, s3, s4, l such that s3 ≤ s1 and s4 ≤ s1 and 0 ≤ l and l ≤ 1 holds (1− l) · s3 + l · s4 ≤ s1. (3) If n > 0, then mn mod m = 0. (4) If j > 0 and i mod j = 0, then i÷ j = j . (5) If n > 0, then in÷ i = in i . (6) If 0 < n and 1 < r, then 1 < rn. (7)...

Three small and distinct satellite double-stranded RNAs (dsRNAs) denoted s1, s1', and s2 were recently described for a Trichomonas vaginalis isolate harboring a dsRNA virus. Since characterization of these satellite dsRNAs might provide insight into the virus replication cycle and virus-host interactions, full-length cDNAs to s1 and s1' dsRNAs were synthesized and sequenced. s1 dsRNA has 688 bp...

We have begun the molecular characterization of genes encoding napin, the 1.7 S embryo-specific storage protein of Brassica napus. Genomic Southern blot analysis indicates that napin is encoded by a multigene family comprised of a minimum of 16 genes. Two DNA fragments containing single napin genes have been recovered from B. napus genomic libraries. We have determined the nucleotide sequence o...

Rat and human ribosomal RNA gene fragments in supercoiled plasmids were examined for S1 nuclease hypersensitivity. In the transcribed portion of genes the number and distribution of S1 sites were found to be species specific. No S1 sites were detected in the promoter regions. In the nontranscribed spacer (NTS), downstream of the 3' end of 28S RNA gene, S1 sites appear to be conserved in rat and...

Infectious bronchitis (IB) is an acute and contagious disease of poultry. The spike glycoprotein (S) of IB virus is a dimmer and is cleaved into two glycopolypeptides, S1 and S2 post-translationally. S1 gene defines the serotype and plays a major role in induction of protective immunity. Eukaryotic expression systems are frequently employed for the production of recombinant S1 proteins as it is...

We have analyzed the prominent supercoil-dependent S1 nuclease cleavage site 5' to hsp 26 in the plasmid 88B13, which contains 11.7 kilobases from the Drosophila locus 67B1. The double stranded cleavage product is generated by initial nicking on the purine strand, six preferred sites occurring between positions -96 and -90 (relative to the start of transcription) with weaker ones extending to p...

A 7 kb chromosomal DNA fragment from R. melilotii was cloned, which complemented temperature-sensitivity of an E. coli amber mutant in rpsA, the gene for ribosomal protein S1 (ES1). From complementation and maxicell analysis a 58 kd protein was identified as the homolog of protein S1 (RS1). DNA sequence analysis of the R. melilotii rpsA gene identified a protein of 568 amino acids, which showed...

Departament de Qúımica Inorgànica, In Universitat de València, 46980 Paterna, Va es; [email protected] National High Magnetic Field Laboratory Florida 32310, USA Dipartimento di Chimica e Tecnologie Chi Arcavacata di Rende, Cosenza, Italy Fundació General de la Universitat de Valè † Electronic supplementary information ( characterization data. Crystallographic (Fig. S1–S23) and tables (Tables S1–...

Abstract The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest ~ 300 amino acid receptor binding domain (RBD) within S1 subunit spike protein because its key interaction with human angiotensin converting enzyme 2...