The genetic structure of 10 populations (453 individuals) of stone loach (Barbatula barbatula L.), a small bottom-dwelling cyprinid fish, in the littoral zone of Lake Constance, central Europe, was investigated by analysing the mitochondrial control region sequences and five microsatellite loci. An unexpectedly high degree of genetic diversity (up to 0.36%) and old estimated age of these popula...

The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30 degrees C for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (dimethyl sulfoxide (DMSO)...

A new species of hillstream loach Balitora eddsi is described from the Karnali River drainage in south-western Nepal. The new species is distinguished from all its congeners by possessing the following combination of characters: six to seven unbranched pectoral-fin rays, pelvic-fin length 12-14% standard length (L(S)), dorsal surface without circular or irregular shaped dark blotches, snout poi...

Loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species' distributions are critical for prioritizing conservation efforts. We developed quantitative PCR assays for detecti...

One of the main goals in current evolutionary biology research is to identify genes behind adaptive phenotypic variations. The advances in genomic technologies have made it possible to identify genetic loci behind these variations, also concerning non-model species. This thesis investigates the genetics of the behaviour and other adaptive traits of the nine-spined stickleback (Pungitius pungiti...

In the last 20 years, new species, asexual reproduction, polyploidy and hybridization have all been reported within the genus Cobitis. An understanding of the current distribution and baseline phylogeographical history of 'true' nonhybrid Cobitis species is crucial in order to unravel these discoveries. In the present work, we investigated the phylogeography of the spined loach, Cobitis taenia,...

Predators have played a significant role in the evolution of herbivorous insects, and we can observe a wide variety of larval defense mechanisms in nature, especially among members of the Lepidoptera. Slug caterpillars (Limacodidae) are known for their unusual morphologies, including various types of protuberances and stinging spines on their dorsal surfaces, which suggest that their evolution ...

a detailed description of the osteological structure of kiabi loach ( oxynoemacheilus kiabii ), a recently described endemic species caught from gamasiab river, iran, is provided. seven specimens were collected by electrofishing in june 2013 and cleared and stained and its osteological structures were described. the results showed that o. kiabii can be distinguished from other members of the ge...

The gene, vitellogenin (vtg) was cloned and characterized in the dojo loach (Misgurnus anguillicaudatus), an indigenous freshwater species in East Asia, in order to develop tools for detecting the effects of estrogenic endocrine-disrupting chemicals (EEDCs). Full-length cDNAs encoding seven distinct vtg transcripts (vtg1-7) were obtained. The corresponding deduced amino acid sequences (Vtg1-7) ...

Spinycheek crayfish ( Faxonius limosus ) and signal Pacifastacus leniusculus are successful North American invasive species distributed throughout Europe. Both compete with native benthic fish for shelter. In a laboratory approach, we assessed competition shelter antagonistic interactions between these the species, stone loach Barbatula barbatula bullhead Cottus gobio ). This allows studying po...