Ruminal bacteria or fungi were selected by the addition of cycloheximide or streptomycin and penicillin, respectively, to ruminal fluid, and the weakening and degradation of lignified tissues in alfalfa and Bermuda grass stems by these treatments and whole ruminal fluid were evaluated in vitro. Dry weight loss in alfalfa was similar for whole ruminal fluid and streptomycin-penicillin treatment,...

There is limited knowledge about the impact of long-term feeding a high-concentrate (HC) diet on rumen microbiota, metabolome, and host cell functions. In this study, a combination of mass spectrometry-based metabolomics techniques, 454 pyrosequencing of 16S rDNA genes, and RT-PCR was applied to evaluate the changes of ruminal microbiota composition, ruminal metabolites, and related genes expre...

Uptake and transport of Zn from (65)Zn-labeled ZnSO(4) and Zn proteinate (ZnProt) by ruminal and omasal epithelia were examined by using a parabiotic chamber system. Uptake was measured during a 4-h incubation with 10, 20, or 200 microM Zn as ZnSO(4) or ZnProt in the mucosal buffer (pH 6.0, Krebs-Ringer phosphate). Zinc uptake and transport were also evaluated after simulated ruminal digestion....

Dietary supplementation of alfalfa hay or calf starter during the preweaning period was beneficial to gastrointestinal development in dairy calves and lambs. In present study, we designed 2 experiments using weaning with investigate diet-ruminal microbiome-host crosstalk yak by analyzing ruminal microbiota rumen epithelial transcriptome. During period, either significantly promoted animal growt...

introduction: this study was designed to investigate the characters of the human endometrial epithelial cells when cultured on either plastic or ecm (extracellular matrix) gel surface and to study ultrastructural features of cultured cells in comparison to endometrial tissue. material and methods: human endometrial tissue was obtained from patients who had undergone hysterectomy for uterine myo...

We used 28 crossbred wether lambs to determine the effects of dietary forage:concentrate ratio and metabolizable energy intake on visceral organ growth and oxidative capacity of gut tissues in lambs. Lambs were assigned randomly to a factorial arrangement of dietary treatments consisting of pelleted diets containing either 75% orchardgrass or 75% concentrate fed once daily at either .099 or .18...

Alimet (Novus Inter., Inc., St. Louis, MO) feed supplement (an 88% aqueous solution of 2-hydroxy-4-(methylthio) butanoic acid; HMB) is a source of L-Met commonly used in nonruminants and ruminants. The absorption of HMB across ovine omasal and ruminal epithelia was evaluated in this study. Ruminal and omasal epithelia were collected from eight lambs (BW = 67.6 kg +/- 9.1) and mounted in parabio...

BACKGROUND In ruminants, lower ruminal pH causes massive disruption of ruminal epithelial structure during periods of feeding high-concentrate diets. However, the influence of excessive organic fatty acids in the lumen of hindgut on the epithelial structure is unclear. In this study, twelve mid-lactating goats were randomly assigned to either a HC diet group (65% concentrate of dry matter; n = ...

Sixteen steers averaging 362 kg body weight were used in a 90-day trial to study the effects of feeding ronnel, a systemic grub control insecticide and larvicide, and activated carbon (AC) on tissue ronnel concentration, the systemic potency of ronnel, feedlot performance, diet digestibility and ruminal and blood characteristics. The steers were individually fed a 70% concentrate diet and assig...

Header: Rumen Epithelial Adaptation And Ruminal Acidosis 1 2 3 Bovine Rumen Epithelium Undergoes Rapid Structural Adaptations During Grain4 Induced Subacute Ruminal Acidosis 5 Michael A. Steele, Jim Croom, Melissa Kahler, Ousama ALZahal, Sarah E. Hook, Kees 6 Plaizier, Brian W. McBride 7 Department of Animal and Poultry Science, University of Guelph, Guelph, Canada 8 Department of Poultry Scien...