greenhouse gases have been of serious global concern to environmentalists. enteric ruminal fermentation and manure are seen to be responsible for global warming. based on a better understanding of positive tan-nin effects on ruminant nutrition, the feeding value of browse trees and shrubs containing tannin, their roles on methanogenic rumen microbes in overcoming the production of enteric ferme...

Though hidden from view, the rumen and its microbes hold a central role in feeding of cattle. During ruminal fermentation, microbes break down fiber and other feed components and produce volatile fatty acids (VFA). In the process, microbes generate adenosine-tri-phosphate (ATP, energy) for themselves, then harness part of this ATP to produce microbial protein. The VFA so produced meet up to 70%...

approved: A. Morrie Craig N Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were id...

This experiment was designed to investigate the effect of lerak extract on the dynamic of rumen microbes in the in vitro fermentation of diet with different ratios of forage and concentrate. In vitro fermentation was conducted according to the method of Tilley and Terry (1963). The design of experiment was a factorial block design with 2 factors. The first factor was the ratio of forage and con...

An average of 11 (range, 2 to 47) mumoles of formate per g per hr was produced and used in whole bovine rumen contents incubated in vitro, as calculated from the product of the specific turnover rate constant, k, times the concentration of intercellular formate. The latter varied between 5 and 26 (average, 12) nmoles/g. The concentration of formate in the total rumen contents was as much as 1,0...

Ruminant livestock are important sources of human food and global greenhouse gas emissions. Feed degradation and methane formation by ruminants rely on metabolic interactions between rumen microbes and affect ruminant productivity. Rumen and camelid foregut microbial community composition was determined in 742 samples from 32 animal species and 35 countries, to estimate if this was influenced b...

Cultures of Streptococcus bovis and mixed populations of rumen bacteria were used to investigate the concentration of ATP and rumen bacterial numbers at various stages of growth. ATP, extracted with Tris buffer, was analyzed using the firefly luciferin-luciferase bioluminescent reaction. ATP concentrations of S. bovis and mixed cultures of rumen bacteria significantly correlated with live cell ...

Ruman degradation is crucial in the supply of dietary nutrients to meet the nutrient demands of the anaerobic microbes and body tissues of ruminant animals. Therefore, it is essential to study the dynamics of rumen degradation of various feeds before their potential use to formulate nutritious diets for ruminant animals. Amongst many methods that have been used in the past, the in sacco method ...

The rumen is an open fermentation chamber, inhabited by microorganisms that anaerobically digest complex components of feedstuffs and generate fermentation products (mainly acids) and microbial cell mass for utilization by the host. The absorption of fermentation products, mainly VFA, from the rumen into the blood, and outflow of microbial cells to the abomasum and small intestine provide the a...

Six double-muscled Belgian Blue bulls (initial weight: 345 +/- 16 kg) with cannulas in the rumen and proximal duodenum were used in two juxtaposed 3 x 3 Latin squares to study the effect of a lack of synchronization between energy and N in the rumen on microbial protein synthesis and N metabolism by giving the same diet according to three different feeding patterns. The feed ingredients of the ...