A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...

 Background and objective: Congenital Rubella Syndrom (CSR) could be  prevented if young women knew their immune status befor pregnancy,contributing in this way to decrease the birth morbidity rate due to CRS amonge the children.  Method: In order to determination the level of antibody or immunity to rubella in  rafsanjan city,270 sera of 14-18 years old girls were evaluated reterospectivly.EL...

Background: The rubella outbreak in Japan has not yet been eliminated. In particular, an outbreak of congenital rubella syndrome has recently become a public health problem in Japan. The World Health Organization has set an elimination target of 2015 for measles in Japan. However, an outbreak of measles occurred in Japan between 2007 and 2008. Starting in April 2006, the measles and rubella vac...

To determine the presence of rubella immunity among pregnant women attending their first prenatal visit in Jeddah, Saudi Arabia, a retrospective, descriptive, cross-sectional, hospital-based study (prevalence study) was undertaken. A total of 10276 women attending prenatal clinics between January 1, 2008, and December 31, 2011 were included. Rubella screening tests (immunoglobulins: IgG and IgM...

background and aims: primary infections of rubella and human cytomegalovirus (hcmv) can lead to severe complications in pregnancy. the screening of rubella and hcmv in pregnant women is not routinely carried out in iran. this cross sectional study aimed to investigate the seroprevalence of hcmv and rubella infections in neonates with and without congenital defects. materials and methods: this s...

a dot immunobinding assay ( dia) was used for a quantitative and qualitative assay of rubella antibody. purified antigen and conjugated anti-human immunoglobulin (rahlg) were prepared. nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. the reacting antibodies were visualized by a peroxidase system. development of a colored insoluble substrate was t...

A case of reinfection with the wild rubella virus in the 8th gestational week is reported. The patient had preexisting hemagglutination inhibition antibodies of low titer following immunization with rubella vaccine. Reinfection was accompanied by clinical symptoms and the presence of rubella-specific immunoglobulin M (IgM) of high titer. Following termination of pregnancy no rubella virus could...

Urine and serum samples from 89 healthy volunteers and three healthy individuals who underwent rubella vaccination were tested for immunoglobulin G (IgG), IgA, and IgM to rubella virus (RV) by enzyme-linked immunosorbent assay methods. Subjects with positive (n = 68) or negative (n = 21) results for serum IgG were exactly the same as those with the corresponding results for urinary IgG. Both ur...

A search for antibody rises to viral antigens other than to Epstein-Barr virus, the causative agent, has been carried out in serial serum samples from 82 patients with infectious mononucleosis (IM). Fourfold or greater rises in titer rarely occurred and did did not cluster in time. No rises occurred to cytomegalovirus, only 1.2 percent to herpes simplex virus, and 8.5 percent to varicella zoste...