Micropropagation of Kainth using nodal explants was carried out in tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Per cent necrotic culture was found to be influenced by...

A hydroponic experiment was carried out to investigate the effect of cadmium treatments at (0.3, 0.6, 0.9 and 1.2 mg/l), zinc at (2, 4, 6 and 20 mg/l) and combined cadmium-zinc at (0.6 mg/l Cd plus 2, 4, 6 and 20 mg/l Zn) on rooting, nutrient uptake in cowpea seedling. The results showed that increasing Cd supply markedly reduced the rooting of seedling. Increased Cd accumulation in cowpea seed...

an experiment was conducted to study the micropropagation of satureja khuzistanica. the seeds were planted in 1/2ms medium. after germination and growth of plantlets, they were subcultured in fresh medium. then, they cultured into medium supplemented by different concentrations of ba and kinetin. results showed that, the highest proliferation (2.4 shoots) was observed at concentrations of 0.5 m...

background: stevia rebaudiana bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. objective: the present study was conducted to optimize a protocol for rapid micropropagation of stevia rebaudiana by shoot tip explants and to invest...

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and αnaphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm TDZ and 0.1 mg dm NAA. It was noted t...

Background: Stevia rebaudiana Bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. Due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. Objective: The present study was conducted to optimize a protocol for rapid micropropagation of Stevia rebaudiana by shoot tip explants and to inv...