this study was conducted to assess the preventive effect of aqueous extract of berberis integerrima bge. root (aebir) on liver damage and oxidative stress induced by diabetes mellitus in rats. forty male rats were divided into 5 groups as follows: 1- normal (n); 2- normal + barberry (n+b) (they received barberry root extract for 6 weeks); 3- diabetic (d) (they received streptozotocin (stz), 65 ...

Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...

Root induction by auxins is still not well understood at the molecular level. In this study a system has been devised which distinguishes between the two active auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). IBA, but not IAA, efficiently induced adventitious rooting in Arabidopsis stem segments at a concentration of 10 microM. In wild-type plants, roots formed exclusively ou...

The induction and secretion of acid phosphatases (APases) is a universal response of plants to phosphate (Pi) starvation. AtPAP10 (Arabidopsis purple acid phosphatase 10) is a major Pi starvation-induced APase that is associated with the root surface in Arabidopsis. So far, the roles of local and systemic signalling in regulating root-associated AtPAP10 activity remain largely unknown. In this ...

Using (13)NO(3) (-), effects of various NO(3) (-) pretreatments upon NO(3) (-) influx were studied in intact roots of barley (Hordeum vulgare L. cv Klondike). Prior exposure of roots to NO(3) (-) increased NO(3) (-) influx and net NO(3) (-) uptake. This ;induction' of NO(3) (-) uptake was dependent both on time and external NO(3) (-) concentration ([NO(3) (-)]). During induction influx was posi...

Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids. Objective: To evaluate the hairy root culture as a novel method for thebaine production. Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by...

Different sugarcane varieties (CP 77/400, CP 85/1491 and Punjabi) were checked for its callus induction and regeneration capabilities using different growth regulators and explants (eye bud, upper portion of inner leaf whorls and lower portion of inner leaf whorls). Seven callus induction media i.e. CIM-1 to CIM-6 and control media, five shoot induction media i.e. SIM-1 to SIM-4 and control med...