The aim of this review was to systematically assess the literature on cytotoxicity of root canal irrigants and intracanal medicaments on stem cells of the apical papilla. A structured strategy was employed and 7 papers were identified. The papers were analysed for concentration of agent used, testing method and viability of stem cells of the apical papilla (SCAP). Irrigation protocols using a f...

background and aims. triple antibiotic paste (tap) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. the aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of tap at 1-, 2-, 3-, and 4-week intervals on mature enterococcus faecalis biofilm. materials and methods. a total of 287 extracted one-rooted hu...

Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile huma...

  Objective: In necrotic immature teeth, intra canal medicaments such as triple antibiotic paste (TAP) and calcium hydroxide (CH) are used for root canal disinfection and regeneration treatment. However, the effect of these medicaments on dental pulp fibroblasts has yet to be known. This study aimed to assess the cytotoxicity of CH and TAP against cultured human dental pulp fibroblasts (HDPFs) ...

microorganisms play a major role in initiation and perpetuation of pulpal and periapical diseases. therefore, elimination of the microorganisms present in the root canal system is the fundamental objective of endodontic treatment. the use of mechanical debridement, chemical irrigation or other antimicrobial protocols and intra-canal medicaments are critical to attain this goal. the aim of this ...

AIM To compare the efficiency of removing calcium hydroxide [Ca(OH)(2)]/chlorhexidine (CHX) (gel), Ca(OH)(2)/CHX (solution) and Ca(OH)(2)/saline pastes with the use of instrumentation and irrigation with sodium hypochlorite and ethylene diamine tetraacetic acid (EDTA) solutions. Moreover the role of the patency file in the cleanliness of the apical third of the root canal was evaluated. METHO...

Biofilms and microbial aggregates are the common mechanisms for the survival of bacteria in nature. In other words, the ability to form biofilms has been regarded as a virulence factor. Microbial biofilms play an essential role in several infectious diseases such as pulp and periradicular pathosis. The aim of this article was to review the adaptation mechanisms of biofilms, their roles in pulpa...

Regenerative endodontic treatment was performed on a mature maxillary premolar diagnosed as chronic pulpitis. The root canals were chemomechanically prepared and placed intracanal medicaments at the first appointment. Then 2 weeks later, a blood clot was created in the canals, over which mineral trioxide aggregate was placed. At 6-month follow-up, cementum-like tissue seemed to be formed in the...

INTRODUCTION This study compared the root canal microbial count of necrotic teeth after irrigation with 6% sodium hypochlorite (NaOCl) (single session treatment) and two-session root canal treatment with two-week application of calcium hydroxide (CH) mixed with 0.2% chlorhexidine (CHX) as intracanal medicament. METHODS AND MATERIALS In this randomized clinical trial, single-rooted necrotic te...