RNA interference (RNAi) is a post-transcriptional gene silencing mechanism that widely exists in eukaryotes. RNAi is initiated by double-stranded RNA (dsRNA) or microRNA (miRNA) and degrades specific messenger RNAs (mRNAs) by RNA-induced silencing complex (RISC), and then it specifically impairs the expression of target genes [1,2]. Therefore, RNAi has been becoming an effective tool to explore...

RNA interference (RNAi) was investigated with the aim of achieving gene silencing with diverse RNAi platforms that include small interfering RNA (siRNA), short hairpin RNA (shRNA) and antisense oligonucleotides (ASO). Different versions of each system were used to silence the expression of specific subunits of the heterotrimeric signal transducing G-proteins, G alpha i2 and G beta 2, in the RAW...

If fields of study have birthdays, then RNA interference (RNAi) turned eight on February 19th this year. Even by the standards of 21st century science, RNAi has been precocious. A PubMed search for “RNA interference” retrieves more than 7900 articles, all published after the landmark 1998 Nature paper by Craig Mello at the University of Massachusetts Medical School and Andy Fire, then at the Ca...

Insects and ectoparasites are causes for major concern throughout the world due to their economic welfare impacts on livestock agriculture. Current control measures involve chemicals such as acaricides which pose challenges like chemical resistance longer withholding periods. To enable more sustainable agriculture practices, it is important develop technologies that combine targeted effectivene...

RNA interference (RNAi) is the phenomenon that double-stranded RNA (dsRNA) induces post-transcriptional sequence-specific gene silencing in eukaryote."2] During the process of RNAi, long dsRNA is cleaved into -21 nt small interfering RNAs (siRNAs), and the siRNAs then guide sequencespecific mRNA degradation. Since the discovery in Caenorhabditis etegans,3] RNAi has been shown to eccur in many e...

The RNA interference (RNAi) pathway is found in most eukaryotic lineages but curiously is absent in others, including that of Saccharomyces cerevisiae. We show that reconstituting RNAi in S. cerevisiae causes loss of a beneficial double-stranded RNA virus known as killer virus. Incompatibility between RNAi and killer viruses extends to other fungal species in that RNAi is absent in all species ...

Gene knockdown by RNA interference (RNAi) in Caenorhabditis elegans is readily achieved by feeding bacteria expressing double-stranded RNA (dsRNA). Enhanced RNAi (Eri) mutants facilitate RNAi due to their hypersensitivity to dsRNA. Here, we compare eight Eri mutants for sensitivity to ingested dsRNA, targeting a variety of tissue-specific genes.

RNA interference (RNAi) of target genes is triggered by double-stranded RNAs (dsRNAs) processed by conserved nucleases and accessory factors. To identify the genetic components required for RNAi, we performed a genome-wide screen using an engineered RNAi sensor strain of Caenorhabditis elegans. The RNAi screen identified 90 genes. These included Piwi/PAZ proteins, DEAH helicases, RNA binding/pr...

Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...