Background: Recently, it has been revealed that tyrosine kinase inhibitors (TKIs) act through inducing both oxidative and endoplasmic reticulum (ER) stress in chronic myeloid leukemia cells. However, ER stress signaling triggers both apoptotic and survival processes within cells. Nevertheless, mechanisms by which TKIs avoid the pro-survival effects are not clear. The aim of this study was to ev...

objective(s): β-thalassemia is one of the most common genetic disorders in the world. as one of the promising treatment strategies, fetal hemoglobin (hb f) can be induced. the present study was an attempt to reactivate the γ-globin gene by introducing a gene construct containing klf1 binding sites to the k562 cell line. materials and methods: a plasmid containing a 192 bp sequence with two repe...

background: immunotoxins are comprised of both the cell targeting and the cell killing moieties. we previously established a new immunotoxin, i.e. shiga toxin granulocyte macrophage-colony stimulating factor (stxa1-gm-csf), comprises of catalytic domain of stx, as a killing moiety and gm-csf, as a cell targeting moiety. in this study, the ability of the immunotoxin to induce apoptosis and doubl...

Ouabain has shown powerful anti-proliferation activities in various cancers, but its effect on imatinibresistant chronic myeloid leukemia and toxicity normal mice not been investigated. Cell Counting Kit-8 assay was used to detect cytotoxicity reversal of ouabain with different concentration (0.01 μM, 0.1 1.0 10 μM) drug resistance imatinib-resistant cell line (K562/G01 line). Flow cytometry th...

background and aim: micro rnas are a group of small non-coding rnas which play an important role in multiple processes such as proliferation, differentiation, apoptosis, and cancer. recent studies indicate that mir-210 is overexpressed into erythroid linage during the differentiation of hematopoietic precursor. the main goal of the present study is to investigate the influence of mir-210 on the...

objective: the itpa gene is responsible to remove free deaminated purine nucleotides of itp, ditp and xtp from nucleotide pool of the cells. it seems that dysfunction in its activity, not only can increas the base substitution mutations frequency but also can works as a contrived factor to creating instability in genetic materials of the cells. there are several reports about the existence of s...

Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...

controversial immunomodulatory properties of bee venom (bv) have provided an appropriate field for more investigation. the aim of present research was to verify the effects of honeybee venom on matrix metalloproteinase activity and interferon production as well as cell proliferation in monocyte and fibroblast cell lines. the monocyte and fibroblast cell lines (k562, ht-1080, wehi-164) were used...

Among various treatments, plants play a crucial role in cancer chemotherapy. Base on literature data, different species of the thymeleaeceae family have been found to be good sources for anticancer agents. Therefore, our laboratory has initiated a research project on evaluating the anticancer properties of the Iranian medical plant, Daphne mucronata (Thymeleaeceae). Gnidilatimonoein is a new di...

OBJECTIVE Bone marrow mesenchymal stem cells (BMMSCs) reside in the bone marrow and control the process of hematopoiesis. They are an excellent instrument for regenerative treatment and co-culture with hematopoietic stem cells (HSCs). MATERIALS AND METHODS In this experimental study, K562 cell lines were either treated with butyric acid and co-cultured with MSCs, or cultivated in a conditione...