The Immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of Toxoplasma gondii (RH strain) was investigated. Immunized mice survived challenge infection and displayed increased interferon gamma (INF-γ ) production. On the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to Concanavalin A (Con A). No tachyz...

BACKGROUND Based on recent studies, there are controversial reports on the capacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capacity was evaluated by in vivo and in vitro experiments. METHODS RH strain was subcutaneously inoculated to ten Wistar rats. After one month, their blood, brain, tongue and diaphragm were collected and evaluated by MAT, PCR, pathologi...

Protection against a challenge infection with Toxoplasma gondii VEG strain oocysts was examined in pigs after vaccination with T. gondii RH strain tachyzoites with or without a porcine specific synthetic oligodeoxynucleotides (ODN) containing immunostimulatory CpG motifs. Six groups of pigs were immunized with incomplete Freund's adjuvant (IFA) and either vehicle, tachyzoites alone or in combin...

The risk of blindness caused by ocular toxoplasmosis supports efforts to improve our understanding for control of this disease. In this study, the involvement of CD8(+), CD4(+), B cell, and IL-10 gene in the immune response of primary ocular infection with the temperature-sensitive mutant (ts-4) of the RH Toxoplasma gondii strain, and in the protective immunity of ocular ts-4 vaccination and ch...

The incidence of congenital toxoplasmosis in Fischer rats infected between the 8th and 12th days of pregnancy with three different strains of Toxoplasma gondii (RH, 76K, and Prugniaud) were 58.2, 35.2, and 62.8%, respectively. No infected fetuses were collected from rats previously infected with RH or Prugniaud strain parasites, even if the rats were reinfected during pregnancy. Since pups from...

The incidences of congenital toxoplasmosis in Fischer rats infected between the 8th and 12th days of pregnancy with three different strains of Toxoplasma gondii (RH, 76K, and Prugniaud) were 58.2, 35.2, and 62.8%, respectively. No infected fetuses were collected from rats previously infected with RH or Prugniaud strain parasites, even if the rats were reinfected during pregnancy. Since pups fro...

Phenacoccus solenopsis, the cotton mealy bug, has emerged as a serious pest of cotton in india, necessitating basic studies on its development and survival at different temperatures (20, 25, 30 and 35 °C) and relative humidities (rH) (65, 75 and 85 ± 1 %) levels to ascertain optimal population growth. a life table was constructed using temperature and humidity. The nymphal duration was 20.2 d a...

Stainless steel disks (diameter, 1 cm) were contaminated with fecally suspended hepatitis A virus (HAV; strain HM-175) and held at low (25% +/- 5%), medium (55% +/- 5%), high (80% +/- 5%), or ultrahigh (95% +/- 5%) relative humidity (RH) at an air temperature of 5,20, or 35 degrees C. HAV survival was inversely proportional to the level of RH and temperature, and the half-lives of the virus ran...

Strength, stiffness, strain at fracture and moisture content of onion skins were measured after incubation at a range of humidities. Higher relative humidity (RH) resulted in skins which had greater moisture content, were stronger in multi-directional tests and which were less stiff. In uni-directional tests, skins incubated at higher RH had extended more at fracture than those incubated at low...