Mechanism-based cytochrome P450 inactivation is defined as a time- and NADPH-dependent inactivation that is not reversible upon extensive dialysis. Current methodologies use dilution approaches to estimate the rate of inactivation and offer limited mechanistic insight and are significantly influenced by experimental conditions. We investigated the potential of progress curve analysis because th...

Genetic strategies for perturbing activity of selected neurons hold great promise for understanding circuitry and behavior. Several such strategies exist, but there has been no direct demonstration of reversible inactivation of mammalian neurons in vivo. We previously reported quickly reversible inactivation of neurons in vitro using expression of the Drosophila allatostatin receptor (AlstR). H...

The role of raphe nucleus (R.N) and serotonin in some behaviors such as sleep, cognition, mood, and memory has previously been reported. The median raphe (MR) nucleus is a major serotonin-containing cell group within the brainstem and is one of the main sources of projections to the septum and hippocampus. The hippocampus is widely believed to be essential for context-conditioning learning. Mor...

The role of raphe nucleus (R.N) and serotonin in some behaviors such as sleep, cognition, mood, and memory has previously been reported. The median raphe (MR) nucleus is a major serotonin-containing cell group within the brainstem and is one of the main sources of projections to the septum and hippocampus. The hippocampus is widely believed to be essential for context-conditioning learning. Mor...

The relationship between membrane permeability, changes in ultrastructure, and inactivation in Escherichia coli strain K-12TG1 cells subjected to high hydrostatic pressure treatment at room and subzero temperatures was studied. Propidium iodide staining performed before and after pressure treatment made it possible to distinguish between reversible and irreversible pressure-mediated cell membra...