Non-steroidal anti-inflammatory drugs (NSAIDs) are classified as Class 4 agents by the Association of Racing Commissioners International and are banned in racehorses during competition in Pennsylvania (PA). To control the abuse of these agents in racehorses competing in PA, a forensic method for screening and confirmation of the presence of these agents is needed. Equine plasma (0.5 mL) was aci...

A simple and sensitive high performance liquid chromatographic (HPLC) method was developed for quantification of lacidipine in human plasma. Felodipine was used as an internal standard (IS). The present method used protein precipitating extraction of lacidipine from plasma. Separation was carried out on reversedphase c18 column (250 mm × 4.6 mm, 5μ) and the column effluent was monitored by UV d...

The objective of the current study was to developed simple, precise and accurate isocratic reversedphase stability indicating HPLC assay method and validated for determination of ticlopidine hydrochloride in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on a Phenomenex Luna C8 (2) column (250 mm ́ 4.6 mm i.d., 5 μm particle size) using mobile phase of methanolammon...

Although chemical modification of analytes is a well-accepted adjunct to chromatography, little attention had been paid to the automation of pre-column derivatization techniques. An automated pre-column system would offer advantages when analyses are performed using labile derivatives; and also when the time between injections is long and so staff time spent on achieving maximum analytical outp...

3,bDiaminopyridine (3,4-DAP) is an effective drug in improving neuromuscular transmission. with limited CNS toxicity, compared to its analogue, 4 aminopyridine (4-AP). 3,4DAP is used in the treatment of neuromuscular diseases, such as Lambert-Eaton Syndrome [ 11. A number of HPLC methods for the quantitation of 3,4-DAP and the related 4-AP have been reported. The method of 3,4-DAP quantificatio...

Background/Aim. Carbamazepine is antiepileptic drug widely used for the treatment of epilepsy. Due to low therapeutic index of carbamazepine there is a need for routine measuring its concentrations in biological fluids. The aim of the study was to describe a method for concomitant determination of carbamazepine in the serum and saliva. Methods. Separation of the drug from matrix is achieved by ...

sion. In 5-Hydroxytryptamine in Peripheral Reactions, F de Clerck, PM Vanhoutte, Eds., Raven Press, New York, NY, 1982, pp 163174. 5. Belenduik K, Belenduik W, Freedman DX. Blood monoamine metabolism in Huntington’s disease. Arch Gen Psychiatry 37, 325332 (1982). 6. Stoward PJ. The possible role of 5-hydroxytryptamine in Duchenne muscular distrophy. In ref. 4, pp 141-147. 7. Curzon G, Green AR....

A sensitive and robust method for the determination of five inorganic and organic Se species in human urine by reversed-phase liquid chromatography with mixed ion-pair reagents coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) is described in this paper. A good separation for anionic, cationic and neutral Se species, namely selenate [Se(VI)], selenourea (SeUr), selenomethi...

The resolution of molecular species of triacylglycerols by reversed-phase high-performance liquid chromatography is discussed. Particular attention is given to the choice of stationary phase, mobile phase, injection solvent, detector and the effect of temperature. The influence of molecular structure on retention time is also explained. Triacylglycerols are major components of the human diet, s...

The development of facile and rapid quantification of biologically active biomolecules such as isotretitoin in therapeutic drugs contained in many generic formulations is necessary for determining their efficiency and their quality to improve the human health care. Isotretritoin finds its applications in the maintenance of epithelial tissues. Different processes to date such as normal phase HPL...