A. Introduction B. Extraction of Lipids from Biological Fluids 1. Gangliosides 2. Prostaglandins and other eicosanoids 3. Purification of total lipid extracts 4. Some miscellaneous extraction procedures C. Adsorption Chromatography for the Isolation of Lipid Classes 1. Lipid class or group separations on silica gel 2. Adsorption chromatography with chemically-bonded phases D. Reversed-Phase App...

a simple and sensitive method has been developed for the preconcentration and determination of trace level of co(ii) using uv-vis spectrophotometry. β-cyclodextrin polymer modified with 1-(2-pyridylazo)-2-naphthol (pan) is used for the preconcentration of co(ii) from samples at ph 8.5. the polymer is synthesized and characterized using elemental analysis, ft-ir and sem analysis. the factors aff...

Analytical methods published in the last 20 years for the extraction, chromatographic separation, and quantification of alkylphenol ethoxylates (APEO) and related compounds in environmental samples are reviewed. Examples of various isolation and preconcentration techniques for water, effluent, sediment and sludge are presented. This includes procedures from the classical liquid-liquid and Soxhl...

a chelating resin is prepared by condensation polymerization of aniline with formaldehyde andcharacterized by ft-ir, elemental analysis and studied for the preconcentration and determination of traceplatinum ion from environmental water sample using inductively coupled plasma-atomic emission spectroscopy(icp-aes). the optimum ph value for sorption of the metal ions was 5. the maximum adsorption...

Polymer microfluidic chips employing in situ photopolymerized polymethacrylate monoliths for high-performance liquid chromatography separations of peptides is described. The integrated chip design employs a 15 cm long separation column containing a reversed-phase polymethacrylate monolith as a stationary phase, with its front end seamlessly coupled to a 5 mm long methacrylate monolith which fun...

The techniques of membrane extraction permit the application of classical liquid-liquid extraction (LLE) chemistry to instrumental and automated operation. Various shortcomings of LLE are overcome by membrane extraction techniques as they use none or very little organic solvents, high enrichment factors can be obtained and there are no problems with emulsions. A three phase SLM system (aq/org/a...

Solid-phase extraction (SPE) is an effective tool for the preconcentration of trace elements and their separation from various sample constituents. Octadecyl and other alkyl-bonded silica gels are most widely used for these purposes. The fundamentals of the SPE of inorganic ions are reviewed and compared with those of related techniques (liquid-liquid extraction and reversed-phase liquid chroma...

In the current study, injectable formulations containing Doxercalciferol as the active pharmaceutical ingredient are analyzed by using gradient-elution high-performance liquid chromatography with ultraviolet detection. Various related impurities and degradants are quantified by using solid-phase extraction (SPE) for enhanced sensitivity. The assay of possible related impurities and Doxercalcife...

Extensive preparation of samples before chromatographic analysis is usually the most time-consuming process in the determination of many organic compounds in environmental matrices. In the past, removal of some organic8 from aqueous ,mlution was commonly done by liquid/liquid extraction. However, the introduction of stable, covalently bonded reversed-phase sorbents now allows efficient removal ...