نتایج جستجو برای: reverse transcriptase loop mediated isothermal amplification assay

تعداد نتایج: 876614  

Journal: :Journal of clinical microbiology 2004
Manmohan Parida Guillermo Posadas Shingo Inoue Futoshi Hasebe Kouichi Morita

A one-step, single tube, real-time accelerated reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the envelope gene of West Nile (WN) virus. The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, efficiency, and rapidity under isothermal conditions with a set of six specially designed pri...

2016
Eun-Mi Kim Hyo-Sung Jeon Ji-Jung Kim Yeun-Kyung Shin Youn-Jeong Lee Sang-Geon Yeo Choi-Kyu Park

Here, we describe a uracil-DNA glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that o...

Journal: :Advances in microbiology 2022

As a matter of fact, infectious diseases hamper everyone’s life and produce lifelong threat to everyone neutral age, sex, lifestyle socio-economic status. Nowadays, come into the sight Chikungunya viral (CHIKV) infection injured many Asian African countries, also deliberated in rising countries low countries. CHIKV is positive-sense, enveloped single-stranded, RNA virus belonging genus Alphavir...

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