To perform PCR from serum for the diagnosis of visceral leishmaniasis is convenient and much less invasive than examination deeper compartments such as bone marrow. We compared three Leishmania-specific real-time PCRs with different molecular targets (kinetoplast DNA, small subunit-ribosomal RNA-(ssrRNA-)gene, glucose-6-phosphate isomerase-(gpi-)gene) regarding their sensitivity specificity in ...

(6-4) photolyase is a flavoenzyme that catalyzes the chemically challenging repair of carcinogenic photoproduct lesions in DNA using sunlight. Nature may have overcome challenge by combining two distinct successive photoreactions, each starting with electron transfer from an excited flavin to lesion. We herewith report transient absorption study returns following chemical transformations succes...

In this study, we have assessed the mechanism of cytotoxicity in a series of cisplatin-sensitive and -resistant ovarian carcinoma cells following treatment with equitoxic concentrations of cisplatin. The specific proteolytic degradation and the enzymatic activities of the DNA-dependent protein kinase (DNA-PK) were assessed in the cisplatin-sensitive A2780 cell line and two resistant derivative ...

In this study, we have assessed the mechanism of cytotoxicity in a series of cisplatin-sensitive and -resistant ovarian carcinoma cells following treatment with equitoxic concentrations of cisplatin. The specific proteolytic degradation and the enzymatic activities of the DNA-dependent protein kinase (DNA-PK) were assessed in the cisplatin-sensitive A2780 cell line and two resistant derivative ...

Abstract Background Parvovirus B19 (B19V) and Human Herpesvirus 6 (HHV6) are commonly detected in endomyocardial biopsy (EMB) specimens of patients with myocarditis symptoms. Whether B19V- HHV6-DNA belong to the cardiac bio-portfolio remains unclear [1,2]. Until today, role B19V-/HHV6-DNA presence is doubtful. Both viruses have been myocardium, even independent inflammation. Yet, their contribu...

OBJECTIVE (i) To determine the levels of nuclear DNA damage in freshly isolated and cultured neutrophils from SLE patients (SLE), rheumatoid arthritis patients (RA) and healthy individuals and relate these to the percentage of apoptotic neutrophils. (ii) To assess rates of repair of neutrophil oxidative DNA damage. METHODS The comet assay was used to quantify nuclear DNA damage in neutrophils...

Activation of Adriamycin by formaldehyde leads to the formation of drug-DNA adducts in vitro and these adducts stabilise the DNA to such a degree that they function as virtual interstrand cross-links. The formation of these virtual interstrand cross-links by Adriamycin was investigated in MCF-7 cells using a gene-specific interstrand cross-linking assay. Cross-linking was measured in both the n...

This paper discusses the results of the analysis of the relationship between dose and solid cancer incidence among nuclear workers (males) who worked as liquidators after the Chernobyl accident. Information on this cohort of individuals is available at the regional center of Russian National Medical and Dosimetric Registry operating at the RF State Research Centre-Institute of Biophysics. Medic...