Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...

androgenesis via anther culture or microspore culture is one of the current methods for producing haploid and double haploid plants in maize. to use of this method in maize breeding program it should be able to regenerate enough plantlets. anthers culture usually is carried out indirectly via callus induction and regeneration on at least two different media. in this study a responsive genotype,...

lisiantus is introduced among the 10 top cutting flowers in the world. micropropagation can be used in this plant because of some problems in traditional propagation. in this research, the effects of the growth regulators on the in vitro shoot regeneration and rooting of the shoots obtained from nodal explants were investigated. the effects of the different concentrations of bap and kin on shoo...

some soil physical and chemical characteristics were analyzed in order to reveal the relationship between forest soils and regeneration patches. gorazbon district of kheyrud was selected as a case study for the research area. all regeneration patches were recorded using gps device. to assess the spatial structure and to quantify the metrics of regeneration patches, analyzing of landscape metric...

considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were si...