white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including iran. in the present study, a pair of primers was designed according to the sequence of vp 28 gene of wssv in the genbank. vp28 gene from an iranian wssv isolate (irvp28) was cloned, sequenced and expressed in escherichia coli bl21(de3) strain in order to produce vp28 protein...

background: the over-expression of recombinant proteins in large amount is important for production of therapeutic proteins and structural study. there are several systems for expression of recombinant proteins. one of the most relevant expression systems is escherichia coli (e. coli). although this organism has many advantages, most of recombinant proteins expressed in e. coli hosts form inclu...

background: saccharomyces boulardii (s. boulardii) is the best known probiotic yeast. the genetic engineering of this probiotic strain requires the availability of appropriate mutants to accept various gene constructs carrying different selection markers. as the auxotrophy selection markers are under focus, we have generated a ura3 auxotroph mutant of s. boulardii for use in further genetic man...

conclusion: the newly produced recombinant protein bmk revealed to be immunogenic. moreover, anti-bmk antibodies produced in mice were able to recognize both the recombinant bmk neurotoxin and the one in m. eupeus crude venome. taken together, the molecular characterization and recombinant production of the iranian scorpion m. eupeus venom component can serve as a new probe for further studies ...

background : toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. congenital toxoplasmosis is more important during pregnancy for the first time. in this study we expressed and purified p43 toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. methods : the recombinant pgemex-1 contained toxoplasma p43 codi...

conclusions these results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal s. aureus in rats. it can be recommended for human clinical trial studies. results recombinant lysostaphin at 100 μg/ml concentration showed a significant decrease of the optical density compared to the control samples. the in vivo study demonstrated that a single dose (200 μg/d...

Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...

Recent cytogenetic and molecular investigations have improved our understanding of endometrial stromal tumors, including sarcomas (ESS), and helped redefine their classification into more pathogenetically meaningful categories. Because much more can be gained through such studies, we add information on another 22 ESS examined by karyotyping, PCR analysis, expression array analysis, and transcri...