Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as decoy. the carboxypeptidase to degrade angiotensin II (Ang II) 1-7 improves pathologies cardiovascular disease acute lung injury. To address whether enzymatic activity protective against COVID-19, we investigated effects B38-CAP, an ACE2-like e...

Cloning and expression of the L-phenylalanine dehydrogenase gene, from B. sphaericus in E. coli were done. The gene was cloned in the vector pET16b and transformed into E. coli BL21 (DE3). The functional form of the L-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, Re...

background : leishmaniasis is an endemic disease in 88 countries. reports on leishmania drug resistance are growing in number. the mechanism of unresponsiveness against glucantime in iranian cutaneous leishmaniasis has not yet been characterized. to begin the first step in finding an anti- leishmania chemotherapy, we prepared recombinant l. major ptr1 enzyme and characterized its activity by en...

alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. the ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. a. borkumensis have two genetic systems for alkane biodegradation. the first system is alkane hydroxylase (alk-b1and alk-b2) and the second system is  cytochrome p450....

Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...

Full-length cDNAs encoding macrophage inhibitory factor-2 (MIF-2) were cloned from Teladorsagia circumcincta (TcMIF-2) and Haemonchus contortus (HcMIF-2). TcMIF-2 HcMIF-2 cDNA (342 bp) encoded proteins of 114 amino acids, each which was present as a single band about 16 kDa on SDS-PAGE. Multiple alignments the protein sequences showed homology 84% between HcMIF-2, 54–76% with MIF-2s seven nemat...

Objectives: In present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. Materials and Methods: We have cloned bovine prochymosin gene in methylotrophic yeast, P. pastoris, using pPIC9K as an expression vector. The recombinant plasmid was transformed into the host by electroporation, and it was expre...

In this study, the cloning, purification and initial characterization of carbonic anhydrase (DrCA) enzyme which we consider to be important in resistance physiology from extremely radioresistant bacteria Deinococcus radiodurans is performed. addition, effect increased gamma irradiation doses on pH-related DrCA activity was determined. after radiation treatment showed that continuously by 6 fold...

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...