نتایج جستجو برای: recombinant antigens

تعداد نتایج: 218562  

Journal: :iranian journal of allergy, asthma and immunology 0
mahmood mahmoodian shooshtari suzan finerty rew john morgan

the aim of this work was to see whether tamarin immunisation with recombinant vaccinia virus expressing epstein barr virus latent proteins could prime t cells which were, on activation, able to inhibit the outgrowth of epstein barr virus transformed cells in vitro. the vaccination appeared to be successful as all vaccinated tamarins developed vaccinia lesions. however, the vaccination protocol ...

Journal: :Journal of clinical microbiology 1999
E S Umezawa S F Bastos M E Camargo L M Yamauchi M R Santos A Gonzalez B Zingales M J Levin O Sousa R Rangel-Aldao J F da Silveira

The commercially available diagnostic tests for Chagas' disease employ whole extracts or semipurified fractions of Trypanosoma cruzi epimastigotes. Considerable variation in the reproducibility and reliability of these tests has been reported by different research laboratories, mainly due to cross-reactivity with other pathogens and standardization of the reagents. The use of recombinant antige...

Journal: :Filaria Journal 2007
Rohana Abdul Rahman Cheah Hwen-Yee Rahmah Noordin

BACKGROUND Anti-filarial IgG4 antibody has been shown to be a good marker for detection of lymphatic filaria infection. Previous studies demonstrated that anti-filarial IgG4 assay using BmR1 recombinant antigen was highly specific and sensitive for detection of brugian filariasis. For bancroftian filariasis, an equivalent assay employing recombinant antigen expressed from the ORF of SXP1 gene h...

Journal: :Journal of clinical microbiology 2000
L A Magnarelli J W Ijdo S J Padula R A Flavell E Fikrig

Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Borrelia burgdorferi sensu stricto and Western blot analyses with whole cells of this spirochete were used to test human sera to determine which antigens were diagnostically important. In analyses for immunoglobulin M (IgM) antibodies, 14 (82%) of 17 serum samples from persons who had erythema migra...

Journal: :archives of clinical infectious diseases 0
seyedeh mahya marashiyan department of basic sciences, sari agricultural sciences and natural resources university, sari, iran fatemeh moradian department of basic sciences, sari agricultural sciences and natural resources university, sari, iran geita saadatnia department of biotechnology, iranian research organization for science and technology (irost), tehran, iran; department of biotechnology, iranian research organization for science and technology (irost), tehran, iran. tel/fax: +98-2156276636 majid golkar molecular parasitology laboratory, department of parasitology, pasteur institute of iran, tehran, iran

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

Journal: :Infection and immunity 2001
D Abraham O Leon S Leon S Lustigman

Efforts to control Onchocerca volvulus, the etiologic agent of river blindness, have been limited to vector control and drug treatment to eliminate microfilariae, with no means available to prevent infection. The goal of this study was to develop a vaccine against this infection using recombinant antigens that are expressed in the early larval stages of the parasite. Five recombinant antigens, ...

2011
M Hemmati A Seghatoleslam M Rasti S Ebadat N Mosavari M Habibagahi M Taheri A R Sardarian Z Mostafavi-Pour

BACKGROUND One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M. tuberculosis) genome sequence has led to identification of several antigens that can be utilized for accurate diagnosis and control of TB. The aim of this study was to purify the recombinant M. tuberculosis antigens for the eval...

Journal: :Veterinary research 2005
Ricardo Toshio Fujiwara André Macedo Vale João Carlos França da Silva Roberto Teodoro da Costa Josiane da Silva Quetz Olindo Assis Martins Filho Alexandre Barbosa Reis Rodrigo Corrêa Oliveira George Lins Machado-Coelho Lilian Lacerda Bueno Jeffrey Michael Bethony Glen Frank Evaldo Nascimento Odair Genaro Wilson Mayrink Steven Reed Antonio Campos-Neto

Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine...

Bahram Kazemi, Mehdi Shadnoush Mojgan Bandehpour, Negar Seyed Parviz Pakzad

Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  Serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...

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