Cell adhesion and spreading require integrins-mediated cell-extracellular matrix interaction. Integrins function through binding to extracellular matrix and subsequent clustering to initiate focal adhesion formation and actin cytoskeleton rearrangement. Lipid raft, a liquid ordered plasma membrane microdomain, has been reported to play major roles in membrane motility by regulating cell surface...

We propose a Byzantine Fault Tolerant variant of the Raft consensus algorithm, BFTRaft, inspired by the original Raft[1] algorithm and the Practical Byzantine Fault Tolerance algorithm[2]. BFT Raft maintains the safety, fault tolerance, and liveness properties of Raft in the presence of Byzantine faults, while also aiming towards to Raft’s goal of simplicity and understandability. We have imple...

Despite the importance of cholesterol in the formation and function of caveolar microdomains in plasma membranes, almost nothing is known regarding the structural properties, cholesterol dynamics or intracellular factors affecting caveolar cholesterol dynamics. A non-detergent method was employed to isolate caveolae/raft domains from purified plasma membranes of murine fibroblasts. A series of ...

The effect on raft foundation of a barrage due to varying stratum has been examined using finite element analysis. The raft foundation is completely resting on soil media and surrounded by soil and rock media. Eight noded brick elements have been used for three-dimensional modeling and meshing of the rock, soil, cut-off, pier, beam and abutment but raft foundation has been meshed using plate el...

The lipid raft hypothesis proposes lateral domains driven by preferential interactions between sterols, sphingolipids, and specific proteins as a central mechanism for the regulation of membrane structure and function; however, experimental limitations in defining raft composition and properties have prevented unequivocal demonstration of their functional relevance. Here, we establish a quantit...

Some transmembrane proteins must associate with lipid rafts to function. However, even if acylated, transmembrane proteins should not pack well with ordered raft lipids, and raft targeting is puzzling. Acylation is necessary for raft targeting of linker for activation of T cells (LAT). To determine whether an acylated transmembrane domain is sufficient, we examined raft association of palmitoyl...

Spatially restricting cAMP production to discrete subcellular locations permits selective regulation of specific functional responses. But exactly where and how cAMP signaling is confined is not fully understood. Different receptors and adenylyl cyclase isoforms responsible for cAMP production are not uniformly distributed between lipid raft and non-lipid raft domains of the plasma membrane. We...

Integrin alpha(v)beta(3) is overexpressed on neoendothelial cells and frequently on tumor cells. We have developed a peptide-like scaffold (regioselectively addressable functionalized template, RAFT), which holds four cyclo(-RGDfK-) (cRGD) motifs and proved that this molecule (called regioselectively addressable functionalized template-arginine-glycine-aspartic acid, RAFT-RGD) targets integrin ...

Study on the seismic behavior of piled rafts and pile groups while the same amount of construction material and excavation is used in their construction, are the main objective of this research. The process where the raft interaction with soil can affect the seismic response and stress distribution is also discussed in the current study. By means, ABAQUS software was applied for the finite elem...