A simple, direct radioimmunoassay for cortisol in human serum and plasma is described. An antiserum, raised in sheep to a cortisol-3-(O-carboxymethyl)oxime/bovine serum albumin conjugate, is coupled to microcellulose. No extraction is required because plasma samples and standards are incubated with the antiserum and an 125I radioligand in a low-pH buffer, which denatures cortisol-binding globul...

Serum triiodothyronine (T(3)) has been measured by radioimmunoassay and corroborated by analysis of the identical samples with a previously described gas-liquid chromatographic technique. Special features of the radioimmunoassay procedure which permit determinations in unextracted serum include the use of a T(3)-free serum preparation for the construction of the standard curve and of tetrachlor...

A radioimmunoassay for bleomycin has been produced using 125l-labeled bleomycin and antisera raised in rabbits against a carbodiimide-catalyzed bleomycin-bovine serum albumin conjugate. 125l-Labeled bleomycin was synthesized by direct iodination of the drug using the chloramine-T technique. The standard curve of the assay was linear on a logit-log plot and the lower limit of sensitivity was 250...

Human serum immunoglobulins are most frequently measured by single gel diffusion. Radioimmunoassay by use of antibodies bonded to particles is faster and more sensitive, precise, accurate, and specific. We adopted a solid-phase radioimmunoassay (SPRIA) technique, developed in our laboratory, to measure serum. lgG, and compared the results with those of a gel-diffusion method commonly used to me...

A newly developed radioimmunoassay was used to measure the concentration of vancomycin in 137 specimens of serum from patients being treated with this antibiotic. Of these sera, 84 were also analyzed with a microbiological assay technique for vancomycin. Duplicate determinations were done with each of the techniques. Individual values and averaged values for both methods were used for statistic...

introduction: neuropeptide y (npy) is the most abundant peptide present in mammals' brains. the majority of npy-producing neurons is in arcuate and paraventricular nuclei of hypothalamus which affect hypophysiotrapic factors secretion. in this study we investigated the effect of intravenous injections of various doses of npy on thyroxine (t4) and triiodothyronine (t3) serum concentrations....

The radioimmunoassay for nortriptyline described here can detect as little 1 microgram/liter of plasma. Within-day precision and day-to-day precision (CV) were +/- 6 and +/- 11%, respectively, over the concentration range 100-200 microgram/liter. The major metabolite hydroxy-nortriptyline, does not cross react with the antiserum. Results so obtained correlate closely with results by a double-is...

We describe double-antibody procedures for determining alpha-fetoprotein in human serum. An equilibrium procedure can be done in 24 h with a sensitivity of at least 4 mug/liter and coefficient of variation of 5.5%. There are no interferences from normal human sera or sera with certain commonly seen chemical abnormalities. We also describe and discuss sequential procedures that range in sensitiv...