Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 diffe...

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and wi...

Chronic wasting disease detection in the lymph nodes of free-ranging cervids by real time quaking-induced conversion" (2014). Abstract 23 Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of deer, elk 24 and moose, is the only prion disease affecting free-ranging animals. First identified in northern 25 Colorado and southern Wyoming in 1967, new epidemic foci of the disea...

Website fingerprinting (WF) is a well-known threat to users' web privacy. New Internet standards, such as QUIC, include padding support defenses against WF. Previous work on QUIC WF only analyzes the effectiveness of when users are behind VPN. Yet, this not how most browse Internet. In paper, we provide comprehensive evaluation QUIC-padding-based directly web, i.e., without VPNs, HTTPS proxies,...

New services with low-latency (LL) requirements are one of the major challenges for envisioned Internet. Many optimizations targeting latency reduction have been proposed, and among them, jointly re-architecting congestion control active queue management (AQM) has particularly considered. In this effort, Low Latency, Loss Scalable Throughput (L4S) proposal aims at allowing both Classic LL traff...

Abstract Built on top of UDP, the recently standardized QUIC protocol primarily aims to gradually replace TCP plus TLS HTTP/2 model. For instance, HTTP/3 is designed exploit QUIC’s features, including reduced connection establishment time, multiplexing without head line blocking, always-encrypted end-to-end security, and others. This work serves two key objectives. Initially, it offers first ou...

A major unsolved issue of prion biology is the existence of multiple strains with distinct phenotypes and this strain phenomenon is postulated to be associated with the conformational diversity of the abnormal prion protein (PrP(Sc)). Real-time quaking-induced conversion (RT-QUIC) assay that uses Escherichia coli-derived recombinant prion protein (rPrP) for the sensitive detection of PrP(Sc) re...

In vitro propagation of rose (Rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

An essential strategy expected to reduce the global burden of chronic and cardiovascular disease is evidence-based policy. However, it is often unknown what specific components should constitute an evidence-based policy intervention. We have developed an expedient method to appraise and compare the strengths of the evidence bases suggesting that individual components of a policy intervention wi...