نتایج جستجو برای: quantitative rt pcr
تعداد نتایج: 481030 فیلتر نتایج به سال:
مقدمه و هدف: پروتئین وابسته به آگوتی(agrp) یک پپتید اشتها آور قوی است که به میزان زیادی از بخش میانی هستههای کمانی هیپوتالاموس ترشح میشود و در کنترل مرکزی دریافت غذا و اشتها نقشی مهم دارد. مشخصشده که این پپتید در کنترل وزن انسان نقش بسزایی ایفا میکند و سطوح پلاسمایی آن در شرایط گرسنگی و چاقی افزایشمییابد. هدف تحقیق حاضر، بررسی یک دوره تمرین بیهوازی دایرهای بر بیان ژنagrp لنفوسیت کشتیگ...
PURPOSE Accurate testing of HER2 is centrally important for breast cancer therapy and prognosis. Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) are current standard testing methods. As a potential alternative for assessment of HER2, we explored quantitative real-time reverse transcription-PCR (RT-PCR), a fast and inexpensive method yielding quantitative results insensi...
We evaluated a highly sensitive quantitative real-time one-step reverse transcription-PCR (RT-PCR) for detection of human cytomegalovirus pp67 transcripts in monitoring of solid-organ transplant recipients. Results were compared with those of pp65 antigen testing and quantitative DNA-PCR. Due to a low clinical sensitivity, the pp67 RT-PCR was not able to replace these assays.
The analysis of cytokine profiles plays a central part in the characterization of disease-related inflammatory pathways and the identification of functional properties of immune cell subpopulations. Because tissue biopsy samples are too small to allow the detection of cytokine protein, the detection of mRNA by RT-PCR analysis is often used to investigate the cytokine milieu in inflammatory lesi...
Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and ammonia monooxygenase gene expression in situ. The inhibitory effect of RT on the PCR was removed with increasing template concentrations beyond 10(5) to 10(6) copies. Including T4 gene 32 protein during the reverse trans...
manipulation of stress related transcription factors to improve plant stress tolerance is a major goal of current biotechnology researches. myc2 gene encodes a key stress-related transcription factor involved in jasmonate (ja) and abscisic acid (aba) signaling pathways in arabidopsis. brassica napus, as a globally important oilseed crop, is a close relative of arabidopsis. in the present study...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید