The mud crab, Scylla paramamosain, has abundant nutrients in the ovary, where numerous lipids accumulate during ovarian maturation. However, mechanism behind accumulation of ovary crab maturation is largely unknown. This study conducted a comparative transcriptome analysis ovaries crabs at various stages A total 63.69 Gb clean data was obtained, with Q30 93.34%, and 81,893 unigenes were identif...

High-throughput amplicon sequencing that primarily targets the 16S ribosomal DNA (rDNA) (for bacteria and archaea) Internal Transcribed Spacer rDNA fungi) have facilitated microbial community discovery across diverse environments. A three-step PCR utilizes flexible primer choices to construct library for Illumina has been applied several studies in forest agricultural systems. The protocol, whi...

Wheat (Triticum aestivum L.) is one of the most important crops cultivated worldwide. Identifying the complete transcriptome of wheat grain could serve as foundation for further study of wheat seed development. However, the relatively large size and the polyploid complexity of the genome have been substantial barriers to molecular genetics and transcriptome analysis of wheat. Alternatively, RNA...

This paper extends the classical exhaustible-resource/stock-pollution model with the irreversibility of pollution decay. Within this framework, we are wondering first how the potential irreversibility of pollution affects the extraction path. Our aim is also to emphasize the conditions under which the economy will optimally adopt a reversible policy. Once the situation has turned irreversible, ...

The H9N2 subtype low pathogenic avian influenza is one of the most prevalent avian diseases worldwide, and was first documented in 1996 in Korea. This disease caused serious economic loss in Korea's poultry industry. In order to develop an oil-based inactivated vaccine, a virus that had been isolated in 2001 (A/chicken/Korea/01310/2001) was selected based on its pathogenic, antigenic, and genet...

PT. Candi Loka Jamus switched to using micro hydro power plants meet its electrical energy needs because of the high operational costs and environmental impact fuel oil plants. However, during dry season, plant is unable produce enough needs, which could lead damage components decreased performance. To address this issue, potential was calculated time simulation season simulation. The rain data...

Abstract Background There is a growing interest in transcriptomics studies parallel to the advancement of transcriptome databases and bioinformatics, which provided opportunity study responses growths, stimuli stresses. an increase demand for excellent RNA extraction techniques. General protocols can be used extraction, but quality quantity vary different types tissues from organisms. Hence, sp...