Three indicator displacement assays are described for the detection of phosphatidylserine in a bilayer membrane. A series of Zn-dipicolylamine coordination compounds are used to bind selectively to the phosphatidylserine and act as a colorimetric chemosensing ensemble when combined with the UV-Vis indictor pyrocatechol violet. A similar displacement assay uses a coumarin methylsulfonate derivat...

A simple, rapid and sensitive spectrophotometric method for the assay of certain adrenergic drugs, [pyrocatechol (PC), levodopa (LD), methyldopa (MD) and dopamine (DP)] is described. The method involves the oxidation of o-dihydroxybenzene derivatives by K2CrO4 followed by oxidative coupling with sulfanilic acid (SPA), leading to the formation of a red or violet colored product having maximum ab...

Phosphate, 3 - 10 nmol, in 1 dm3 of natural-water samples was quantitatively collected along with 10 micromol of Fe(III) ion onto a silica-gel collector in the pH range of 5.4 - 6.2. The amount of Fe(III) ion needed was limited to such a low level that the Pyrocatechol Violet method could be applied without removing the Fe(III) ion, providing a superior determination method for phosphate. Surfa...

Chick embryo collagen-synthesizing polysomes were isolated by differential centrifugation. RNA extracted from these particles was chromatographed in oligo(dT)-cellulose solumns and the mRNA thus obtained characterized as collagen mRNA by its electrophoetical mobility in acrylamide gels (equivalent to 1.05 x 10-6 daltons) and its effect upon a cell-free system derived from Krebs ascites tumor c...

In this research, the dinuclear Zn(ii) complex of anthracene based tripodal tetramine Zn2L was synthesized, and its sensing abilities towards anions was investigated using the indicator displacement assay (IDA) approach with four complexometric indicators: pyrocatechol violet (PV), bromopyrogallol red (BPG), methylthymol blue (MTB) and xylenol orange (XO). UV-vis spectrophotometry results indic...