Cryopreservation by droplet vitrification was applied to hazelnut (Corylus avellana L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from in vitro growing shoots, immersed ice cooled PVS2 or PVS3 for 60 90 min, then transferred a solution, placed on strip aluminium foil, and plunged into liquid nitrogen (LN). Additionally, effect recovery mother pl...

Development of protocols for cryopreservation microalgae Chlorococcum dissectum Korshikov and Dunaliella salina Teodoresco requires selection effective cryoprotectants their concentrations. One the stages choosing optimal concentrations is determining degree toxicity cells at equilibration stage. Solutions dimethyl sulfoxide, ethylene glycol, ethanol, glycerol (5–30%) modified PVS1 PVS2 (50 75%...

The mangabeira (Hancornia speciosa Gomes) is a fruit tree of high potential that distributed in several regions the Brazil, but which great social economic importance for Northeast region. Due to wide possibility using its fruits can be consumed natura or industrialized, and represent financial source traditional communities practice extractivism this species. objective study was evaluate effic...

Ammodendron persicum (Bunge & Boiss.)is a desert shrub specie which grows on some sand dunes and sandy areas of South Khorasan and Sistan-va-Baluchestan provinces in east of Iran. Low distribution and narrow ecological range have put this species under threat. In order to evaluate the possibility of long-term preservation of A. persicum seeds in cryogenic conditions (-196°C), the seeds of speci...

In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were pre...

Preservation methods were evaluated forjerusalem artichokes (Helianthus tuberosus L.) to facilitate the back-up of field collections. In vitro plant cultures were established from field-harvested tubers for nine jerusalem artichoke cultivars obtained from the Plant Gene Resources of Canada, Saskatoon Research Center, Saskatoon, Canada. Most cultivars could be maintained at 5 °C or under 25°C gr...

achillea millefolium belonging to the astreaceae family is an endangered medicinal plant of jordan and of its neighboring countries. as an alternative to seed propagation, an efficient micropropagation of a. millefolium and its subsequent rooting were developed as an option for its in vitro conservation. a maximum of 5.9 shoots per microshoot were obtained on murashige and skoog agar medium sup...

Cryopreservation of plant tissues in liquid nitrogen is now used for long-term conservation of vegetatively-propagated crops. Development of standard techniques for cryopreservation is important to the international plant-conservation community for successful implementation of storage protocols in diverse and internationally dispersed laboratories. Evaluation of the critical points of each pres...

This study was designed to determine the response of diverse mint genotypes to three commonly used cryopreservation techniques. Four mints [Mentha x piperita nothosubsp. citrata (Ehrh.) Briq.; M. canadensis L.; M. australis R. Br, and M. cunninghamii Benth] were cryopreserved using three protocols: controlled rate cooling (CC), encapsulation dehydration (ED) and PVS2 vitrification (VIT). Regrow...