two peroxidases (ec. 1.11.1.7), pod i and pod ii were purified from the roots of cultivatedraphanus sativus l. var. cicil by one step ion-exchange chromatography after fractionation by acetone. the molecular weight of these enzymes were 43000 and 41000 daltons and rz 1.2 and 2.0 for pod i and pod ii, respectively. both enzymes consisted of a single polypeptide chain on sds-page. the maximum act...

transferrin acts as a vehicle for iron transportation between the absorption, storage and utilization sites in the body and, besides iron, its two highaffinity iron-binding site are capable of binding to 29 other elements. in physiological and pathophysiological conditions, transferrin’s properties are different and close attention to these properties is required. for further investigation for ...

Available online 1 April 2009

Treatment of domestic wastewater, at both the village and urban level, remains a problem in most developing countries. This is a review paper illustrating the use of duckweed-based wastewater treatment systems that provide genuine solutions to these problems. They are inexpensive to install as well as to operate and maintain. They do not require expensive technologies. Therefore, this review pa...

A number of years ago the Green Chemistry Centre of Excellence at York discovered new high surface area forms of polysaccharides. Further study of these materials has found that after controlled thermal treatment they exhibit remarkable thermal properties, which have enabled us to make an entirely new class of materials with properties ranging from starch-like to carbon-like (see figure 1). Tra...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

flocculation processes during estuarine mixing can reduce the elemental concentrations of river water. in the present investigation, natural flocculation processes concept is used to reduce the elemental contents of industrial wastewater. for this purpose, various amounts of saline water were added to an artificial industrial wastewater with known concentrations of heavy metals. the results of ...

background and objectives : streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of streptococcus equisimilis strain h46a. the objective of the present study was the production of streptokinase from strain h46a and purification by chemical reduction method. materials and methods: the rate of...

background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...