Oocyte cryopreservation is of key importance in the preservation and propagation of germplasm. Interest in oocyte cryopreservation has increased in recent years due to the application of assisted reproductive technologies in farm animals such as in vitro fertilization, nuclear transfer and the need for the establishment of ova/gene banks worldwide. However, the cryopreservation of the female ga...

Mouse oocytes were placed in Hepes-T6 containing 7.5 μg/ml cytochalasin B and 5μg/ml Hoechst prior to enucleation, and enucleation was performed on oocytes using the manipulator. The oocyte held in place by gentle suction of a holding pipette (oocyte diameter approx.70 μm). The metaphase spindle (arrow) is aspirated into an enucleation pipette (1).

Solemn meeting 4/I in memory of Pasteur (together with the Kazan Society Naturalists, Scientific Assemblies Doctors Kaz. Clin. Institute and Veterinary Physicians).Prof. V.S.Gruzdev. Life Writings Louis Pasteur. (With screen demonstrations).

Curved quantum waveguides are known to bind particles. We show that a number of charged fermions in such a trap can be tuned by an external electrostatic eld; if the latter is slowly increased, the bent duct can serve as a single particle ejector.