An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare pur...

Being one of the most commonly used electrochemical mediators for analytical applications, Prussian Blue has found a wide use in the biosensor field during the last years. Its particular characteristic of catalysing hydrogen peroxide reduction has been applied in the construction of a large number of oxidase enzyme-based biosensors for clinical, environmental and food analysis. By modifying an ...

The aim of the present study was to observe the in vivo targeting characteristic of angiopoietin 2-small interfering RNA (Ang2-siRNA) plasmid/chitosan magnetic nanoparticles in an established nude mouse model of malignant melanoma (MM) under an external magnetic field. The nude mouse MM model was first established, then divided into 3 groups, including the control group, the non-targeting group...

The timing of the breakdown of red blood cells and organization of hemorrhage has significance in the catabolism of heme and the processing of iron, but also has a practical application in terms of assigning, or attempting to assign, a time course with respect to traumatic events (e.g. contusions and hemorrhages). Attempts to date contusions, however, have generally been unsuccessful by macrosc...

The Electrochemical Society Interface • Fall 2002 olecular magnetism is an evolving discipline relying on quantum chemistry, orbitals and Hamiltonians, organic and coordination chemistries, physics, and devices. Among the exciting molecule-based magnetic systems, Prussian blue analogues occupy a peculiar position. Prussian blue itself was described for the first time in 1704. Since this time, t...

We experimentally show that the magnetocaloric properties of molecule-based Prussian blue analogues can be adjusted by controlling during the synthesis the amount of intrinsic vacancies. For CsxNi II 4 [Cr (CN)6](8+x)/3, we find indeed that the ferromagnetic phase transition induces significantly large magnetic entropy changes, whose maxima shift from∼ 68 K to∼ 95 K by varying the number of [Cr...

The effect of pressure up to 175 kilobars on the electronic structure of three ferric hydroxamates and on ferrichrome A has been studied by optical absorption and Mössbauer resonance. The ferric ion was reduced to ferrous ion with pressure, as has been previously observed for various compounds. For the hydroxamates, the amount of reduction correlated very well with the location and shift of the...