Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation. In order to reveal the lethal an...

Technique for rapid mass propagation of Geodorum densiflorum (Lam)Schltr. has been developed by using thin sections of stems of in vitro regenerated plantlets as explant source. Thin sections of stems (1.0-1.5 mm) when cultured in modified liquid and semisolid Knudson C (KnC) medium produced 1.8 and 1.2 protocorm like bodies (PLBs) per explant respectively. Peptone (2 g l-1) was effective in pr...

The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem's Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusA gene expres...

orchids are one of the most popular plants in the world and among them phalaenopsis genus have the most sales in a global market. because of its hard propagation, micropropagation technique has been employed recently. in this study, we tried to find out useful protocol for commercial production through in vitro culture. at first, for seed capsules production, flowers were pollinated with hand i...

The immature seeds of Dendrobium nobile acquire resistance to freezing and long-term storage in a cryobank at the age 6 months after pollination. Seed maturation Lindl establish optimal stage for liquid nitrogen was studied. Immature can germinate vitro starting from 3 pollination (MAP) but only develop up embryo swelling or protocorm without rhizoids. maximum staining embryos with vital dyes F...