this experiment was carried out to investigate the effect of adaptation period to oak leave's tannin on rumen ph and ammonia, rumen microbial population, gas production and dry matter true digestibility (ivtdmd) in alamout goat’s rumen (with no previous history of oak leaf consumption) compared with markhoz goat (with previous history of oak leaf consumption). the goats were fed either oak...

The pineapple waste from the industry has contributed to an increase in Malaysia and worldwide every year. A major type of endopeptidase enzymes found is fruit bromelain, stem ananain, comasain. This study aims extract purify protease crown MD2 pineapple. Protease was extracted purified using anion exchange chromatography, gel filtration, desalting before being identified liquid chromatography-...

This study evaluated the use of three solid brewery wastes: brewer's spent grain, hot trub and residual brewer's yeast, as alternative media for the cultivation of lactic acid bacteria to evaluate their potential for proteolytic enzyme production. Initially, a mixture experimental design was used to evaluate the effect of each residue, as well as different mixtures (with the protein content set...

BACKGROUND Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. RESULTS In this study, we investigated the dynamics of the assembly and breakdown of a human IgG(1)κ antibody expressed in plants. Initial s...

Bacillus thuringiensis produces d-endotoxins that require proteolytic processing to become active. The activation of the B. thuringiensis subsp. medellin 28 kDa (Cyt1Ab1) cytolytic toxin by trypsin, chymotrypsin and gut extract from Culex quinquefasciatus larvae was analyzed. The Cyt1Ab1 toxin of B. thuringiensis subsp. medellin was processed by all proteases tested to fragments between 23 and ...

BACKGROUND AND AIM Diabetes mellitus is known to cause neurological disorders due to impaired glucose metabolism involving decreased utilization of glucose by the brain tissues. The mechanisms responsible for failure of glycemic regulation in type-2 diabetes leading to neurological impairment need to be thoroughly elucidated. MATERIALS AND METHODS Type-2 diabetes was induced in albino rat mod...

Background Allergies represent a socioeconomic problem that has affected increasing world population. A range of respiratory allergens are themselves proteases or are associated with proteolytic activity. Bromelain is a mixture of cysteine proteases present in the leaves and fruit Ananas comosus, with several pharmacological applications and is widely used in the food industry. Cases of occupat...

The therapeutic efficiency and safety of the proteolytic enzyme bromelaine obtained from pineapple (Bromelain-POS, Ursapharm GmbH, Saarbrücken, Germany) was evaluated in children under the age of 11 years diagnosed with acute sinusitis. Data from 116 patients from 19 centres located across Germany were analysed in a pharmacoepidemiological cohort study. Patient cohorts were either treated with ...

entomopathogenic fungi produce a variety of degrading enzymes, including proteases, chitinases and lipases, to facilitate their entry through the massive barriers of insect cuticle. isolates of the entomopathogenic fungi vary considerably in their proteolytic activity and virulence. the proteolytic activity of different isolates has been hypothesized to reflect their virulence toward the host. ...