As the prototypical member of the PTP family, protein tyrosine phosphatase 1B (PTP1B) is an attractive target for therapeutic interventions in type 2 diabetes. The extremely conserved catalytic site of PTP1B renders the design of selective PTP1B inhibitors intractable. Although discovered allosteric inhibitors containing a benzofuran sulfonamide scaffold offer fascinating opportunities to overc...

BACKGROUND The purpose of this paper is to compare the effectiveness of use of alkaline phosphatase (ALP) and C-reactive protein (CRP) levels in marking and monitoring the risk of preterm delivery due to infection. MATERIAL/METHODS The study involved 83 patients assigned to groups: Group I (n=43) consisted of patients hospitalized for symptoms of preterm delivery, and Group II (control group ...

Protein tyrosine phosphatase 1B (PTP1B) and T-cell protein (TC-PTP) play non-redundant negative regulatory roles in activation, tumor antigen presentation, insulin leptin signaling, are potential targets for several therapeutic applications. Here, we report the development of a highly potent selective small molecule degrader DU-14 both PTP1B TC-PTP. mediated TC-PTP degradation requires target p...

Five new sesterterpenoids, compounds 1-5, have been isolated from the sponge Hippospongia lachne off Yongxing Island in the South China Sea. The structures of compounds 1-5 were elucidated through extensive spectroscopic analysis, including HRMS, 1D, and 2D NMR experiments. The stereochemistry, including absolute configurations of these compounds, was determined by spectroscopic, chemical, and ...

The interaction of the non-receptor tyrosine kinase, Src, with the cytoskeleton of adhesion sites was studied in nerve growth cones isolated from fetal rat brain. Of particular interest was the role of protein tyrosine phosphatases in the regulation of Src-cytoskeleton binding. Growth cones were found to contain a high level of protein tryrosine phosphatase activity, most of it membrane-associa...

Intracellular signaling by protein tyrosine phosphorylation is generally understood to govern many aspects of cellular behavior. The biological consequences of this signaling pathway are important because the levels of protein tyrosine phosphorylation are frequently elevated in cancer cells. In the classic paradigm, tyrosine kinases promote tumor cell growth, survival, and invasiveness, whereas...

An extracellular phosphatase was purified to homogeneity from the entomopathogenic fungus Metarhizium anisopliae with a 41.0% yield. The molecular mass and isoelectric point of the purified enzyme were about 82.5 kDa and 9.5 respectively. The optimum pH and temperature were about 5.5 and 75 degrees C when using O-phospho-L-tyrosine as substrate. The protein displayed high stability in a pH rang...