protein aggregation

Functional Recombinant Extra Membrane Loop of Human CD20, an Alternative of the Full Length CD20 Antigen

Journal: Iranian Biomedical Journal 2012

Aida Feiz Barazandeh, Ali Jahanian-Najafabadi, Hossein Khanahmad, Mahdi Behdani, Mahdi Habibi Anbouhi, Majid Golkar, Mohammad Ali Shokrgozar, Mohammad Reza Aghasadeghi, Mohsen Abolhassani, Saeid Bouzari,

Background: Targeting of CD20 antigen with monoclonal antibodies has become the mainstay in the treatment of non-Hodgkin's lymphomas and immunotherapeutic depletion of malignant B cells. Accessibility of antigen is one of the crucial factors in development of monoclonal antibodies against this antigen. One major problem in expression of full length CD20 is aggregation and misfolding. Therefore,...

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a study on the mechanism of aggregation of therapeutic reteplase protein by using the monomer-loss model

Journal: :applied biotechnology reports 0

soheyla ershadi hamid rashedi ahmad fazeli

aggregation of pharmaceutical proteins reduces the efficiency and increases the cost of production. it can also lead to the reduced efficacy of drug or cause side effects on the patient’s body. investigating how to create them plays an important role to find agents that prevents the aggregation. this study was allocated for understanding the mechanism of formation of the reteplase protein using...

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nonionic surfactants (dodecyl maltoside and polysorbate 20) effect on light induced aggregation and conformational changes of recombinant human ifnβ_1b

Journal: :iranian journal of pharmaceutical research 0

mohammad reza fazeli department of drug and food control, pharmaceutical sciences research center, faculty of pharmacy, tehran university of medical sciences, tehran, iran. najmeh mahjoubi department of drug and food control, pharmaceutical quality assurance research center, faculty of pharmacy, tehran university of medical sciences, tehran, iran. ahmad fazeli bresearch and development department, zistdaru danesh pharmaceutical company. tehran, iran. rassoul dinarvand department of pharmaceutics, faculty of pharmacy, tehran university of medical sciences, tehran, iran. mohammad reza khoshayand department of drug and food control, pharmaceutical quality assurance research center, faculty of pharmacy, tehran university of medical sciences, tehran, iran. maryam shekarchi food and drug research center, food and drug organization, moh&me, tehran, iran

liquid protein formulations are prone to form aggregates. the effect of nonionic surfactants such as polysorbate 20 (ps 20) and n-dodecyl β-d-maltoside (ddm) on the prevention of aggregation and conformational changes of recombinant human ifnβ-1b (rhifn β_1b) was explored. polysorbate has been used in formulations of protein pharmaceuticals. there have been concerns about using ps 20 due to its...

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Nonionic Surfactants (Dodecyl Maltoside and Polysorbate 20) Effect on Light induced Aggregation and Conformational Changes of Recombinant Human IFNβ_1b

Journal: The Iranian Journal of Pharmaceutical Research 2017

Ahmad Fazeli, Maryam Shekarchi, mohammad Reza fazeli, mohammad Reza Khoshayand, Najmeh Mahjoubi, Rassoul Dinarvand,

Liquid protein formulations are prone to form aggregates. The effect of nonionic surfactants such as Polysorbate 20 (PS 20) and n-Dodecyl β-D-maltoside (DDM) on the prevention of aggregation and conformational changes of recombinant human IFNβ-1b (rhIFN β_1b) was explored. Polysorbate has been used in formulations of protein pharmaceuticals. There have been concerns about using PS 20 due to its...

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