Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability to metabolize N(6)-benzyladenine (BA). Formation of N(6)-benzyladenosine-5'-mo...

In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytochemical reducing potential. Here, we developed an efficient protocol for in vitro micropropagation of a high-value endangered medicinal plant species, Phlomis bracteosa, in order to explore its biogenic potential in biomimetic sy...

Tripleurospermum insularum (Asteraceae) is a critically endangered (CR) insular endemic species in Turkey and facing high risk of extinction. Here, rapid efficient vitro propagation protocol using nodal segments obtained from seedling shoots cultured on Murashige Skoog (MS) basal medium supplemented with different plant growth regulators (PRGs) was developed to conserve T. insularum. Besides, t...

Abstract In vitro-propagated potato plantlets are commonly used in seed tuber production. Four experiments were carried out to identify how the duration of last vitro phase (‘age’) before planting vitro-produced affects early growth and development ex vitro, assessed 10–14 days after planting. Experiments included varying ranges age at (10–40, 15–45, 14–28 14–56 old moment respective experiment...

BACKGROUND The low survival rate of in vitro regenerated Panax ginseng plantlets after transfer to soil is the main obstacle for their successful micropropagation and molecular breeding. In most cases, young plantlets converted from somatic embryos are transferred to soil. METHODS In vitro thickened taproots, which were produced after prolonged culture of ginseng plantlets, were transferred t...

From the rhizoplane of Oryza sativa, vars. Morelos A-88 and Apatzingan, rice plantlets, we isolated two bacterial strains: Corynebacterium flavescens and Bacillus pumilus. By scanning electron microscopy, endophytic bacteria were frequently identified at the base of secondary roots, between the epidermis and the mucilaginous layer. Endophytes were also identified in the intercellular spaces whe...

Kalanchoë daigremontiana reproduces asexually by generating numerous plantlets on its leaf margins. The formation of plantlets requires the somatic initiation of organogenic and embryogenic developmental programs in the leaves. However, unlike normal embryogenesis in seeds, leaf somatic embryogenesis bypasses seed dormancy to form viable plantlets. In Arabidopsis (Arabidopsis thaliana), seed do...

It is of interest to apply plant tissue culture to generate plants resistant to toxic effects of cadmium (Cd) on plant growth. Callus cultures were initiated from leaf explants of micropropagated potato plantlets (Solanum tuberosum L., cv. Iwa) for in vitro selection comprising 18 different Cd treatments varying in Cd exposure timing and duration. Plantlets regenerated from two different lines ...

This study was designed to examine the effects of promoting substances on bud retention, rooting and plantlets survival of rooted cuttings on selected tree species using growth promoting substances. Stem cuttings were treated with rooting-promoting substances, planted in plastic pots filled with top soil and arranged using a completely randomized design (CRD) in a screen house. Stem cuttings of...