Aberrant DNA methylation has been reported as an important phenotype in acute myeloid leukemia. However the clinical significance of methylation changes has not been clear yet. In this study methylation Specific Melting Curve Analysis (MS-MCA) and real time PCR was used to assess the CDKN2B promoter hyper-methylation and gene expression in 59 Iranian acute myeloid leukemia (AML) patients. The i...

alteration in methylation pattern of promoters is associated with tumorigenic events,(1, 2) and differentiation control,(3, 4) in several types of cells in humans.specific hypermethylation in promoter region of some genes exist in different types of neoplastic cells.(5) dna methylation indicates to the addition of a methyl moiety to the cytosine in a cpg dinucleotide. cpg dinucleotides tend to ...

Gastric Cancer (GC) is the second most common cancer in the world and a leading cause of cancer-related mortality. Methylation of promoter CpG islands (CGIs) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. Adenomatous Polyposis Coli (APC) a tumor suppressor gene is inactivated by methylation of prom...

hypermethylation of cpg islands located in the promoter regions of genes is a major event in the development of the majority of cancer types, due to the subsequent aberrant silencing of important tumor suppressor genes. klotho; a novel gene associated primarily with suppressing senescence has been shown to contribute to tumorigenesis as a result of its impaired function. recently the relevance ...

Aberrant DNA methylation has been reported as an important phenotype in acute myeloid leukemia. However the clinical significance of methylation changes has not been clear yet. In this study methylation Specific Melting Curve Analysis (MS-MCA) and real time PCR was used to assess the CDKN2B promoter hyper-methylation and gene expression in 59 Iranian acute myeloid leukemia (AML) patients. The i...

introduction: promoter methylation has an inhibitory effect on gene expression. thus we investigated methylation patterns of p15 gene in esophageal squamous cell carcinoma (escc), one of the most frequent and fetal cancers in the world. material and methods: p15 promoter methylation status of 30 escc and 10 normal specimens was investigated using methylation specific pcr. dna extracted from the...

in the recent years deregulation for micrornas expression pattern have emerged as a possible molecular factor for carcinogenesis. it has been reported that the expression of mir-9 was down-regulated in human gastric adenocarcinoma. to figure out the molecular mechanism of this down regulation, the methylation status in promoters of mir-9 family loci were compared in the human gastric adenocarci...

Hypermethylation of CpG islands located in the promoter regions of genes is a major event in the development of the majority of cancer types, due to the subsequent aberrant silencing of important tumor suppressor genes. KLOTHO; a novel gene associated primarily with suppressing senescence has been shown to contribute to tumorigenesis as a result of its impaired function. Recently the relevance ...

e-cadherin is among tumor suppressor genes which mostly subjects to the down-regulation in squamous cell carcinoma of esophagus (scce). the gene is tightly associated with the tumor invasion and metastasis in multiple human cancers, especially scce. cpg islands’ methylation in the promoter region of e-cadherin is among the mechanisms that have been suggested for the e-cadherin silencing, howeve...