Amino acid analysis of [3H]proline-labeled polyomavirus major capsid protein VP1 by two-dimensional paper chromatography of the acid-hydrolyzed protein revealed the presence of 3H-labeled hydroxyproline. Addition of the proline analog L-azetidine-2-carboxylic acid to infected mouse kidney cell cultures prevented or greatly reduced hydroxylation of proline in VP1. Immunofluorescence analysis per...

The present study analyses changes in nitrogen compounds, amino acid composition, and glutamate metabolism in the resurrection plant Sporobolus stapfianus during dehydration stress. Results showed that older leaves (OL) were desiccation-sensitive whereas younger leaves (YL) were desiccation-tolerant. OL lost their soluble protein more rapidly, and to a larger extent than YL. Enzymes of primary ...

Plants exposed to certain abiotic stress conditions tend to produce the amino acid proline, which acts as an active osmolyte, a metal chelator, an antioxidant, and a signaling molecule. There is increasing evidence that proline accumulates in plants due to a wide range of abiotic stress, in particular high soil salinity and drought. Therefore, proline content is often used as a marker-assisted ...

The transport of proline, 5-oxoproline, and pipecolic acid and their mutual interactions during transport were studied in purified brush-border membrane vesicles from rabbit renal cortex. The presence of a Na’ gradient stimulated the transport of these compounds. 5-Oxoproline was transported by a single system with an apparent K,,, of 20.4 mM. Many neutral amino acids and 6-piperidone-2-carboxy...

The amino acid proline is accumulated in plant tissues in response to a variety of stresses. The existence of two routes for its biosynthesis is well documented. However, little is known about the contribution of each pathway to the accumulation of free proline under stress conditions. In the present study young barley plants were subjected to osmotic stress by treating their roots with 25% pol...

The purpose of the work is to study pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.) legume family (Fabaceae) herb amino acid composition and forage value, introduced in Kuzbass. Eighteen proteinogenic acids were identified raw materials third fourth years introduction. Eight them are irreplaceable ten replaceable. total content differed significantly by amounted 6.266±0.89% year 12.541...

The aim of this study was to determine the content and/or composition of protein, peptide, amino acid, lipid and fatty acid in bacterial douchi (BD) made by different pure starter fermentation. Protein content of BD3, BD5, BD7 and BD8 was significantly higher than that of autoclaved soybean (AS). Lipid content of BD1, BD5 and BD6 was also significantly higher than that of AS. Predominant amino ...

The growth of Listeria monocytogenes ATCC 23074 in defined medium is sensitive to high osmolarity when compared with its growth in complex media, such as brain heart infusion (BHI). The two major contributors to this difference in growth rate are the availability in BHI of the osmoprotectant glycine betaine and peptides. Peptone plays two major roles: firstly as a nutritional supplement for pro...

The tobacco alkaloid nicotine possesses a molecular structure by which it may be related empirically to the amino acid proline. Proline is a-pyrrolidinecarboxylic acid. Nicotine may be considered as an N-methylated proline containing a 3-pyridine rino attached to the alpha position in place of the carboxyl. These similarities have been pointed out by TOTTINGHAM (9) and have been employed by TRI...