Calli of soybean [Glycine max cv. Pershing] were grown on "MS" media supplemented with different levels of KCl and NaH2PO4 for 45 days. The effects of salt stress were evaluated on the production and accumulation of proline and growth content of calli. Proline content increased as growth occurred. The maximum amount of proline and the highest content of growth were observed in calli grown on me...

mdash; Plant regeneration from immature intergeneric embryos through direct callus induction was investigated in the case of hybridization between T aestivum cv Chinese Spring (2n = 6x = 42, AABBDD) and A cristatum 4x with one B chromosome (2n = 4x = 28 + 1 B, PPPP). Twenty-three abnormal immature hybrid embryos were directly cultured on MS solid medium with 2 mg / L 2,4-D to induce calli which...

Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation...

Moso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicated by callus induction for monopodial bamboos, such as Moso bamboo. In the present report, embryog...

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintaine...

<i>Verbascum scamandri</i> Murb. known as "Kazdağı Mullein" is an endemic species in Türkiye and classified endangered (EN) species. The aim of this study to establish efficient callus culture for <i>V. analyze the amounts verbascoside, luteolin, aucubin metabolites calli samples. Leaf explants were cultured on MS medium with cytokinin (BAP, Kin, 0, 0.5, 1, 2, 3 mg/L) auxin (N...

Chrysanthemum morifolium propagation through stem cutting produces weak plants that show delayed anthesis above 20 °C and a reduced flower diameter. Therefore, in this study chrysanthemum plantlets were produced somatic embryogenic from calli to exploit the somaclonal variation for their improvement. Various explants variety Dante yellow cultured on Linsmaier Skoog (LS) medium with various conc...

DNA 5-methylcytosine (5-meC) is an important epigenetic mark for transcriptional gene silencing in many eukaryotes. In Arabidopsis, 5-meC DNA glycosylase/lyases actively remove 5-meC to counteract transcriptional gene silencing in a locus-specific manner, and have been suggested to maintain the expression of transposons. However, it is unclear whether plant DNA demethylases can promote the tran...

The fast development of multi-drug resistant (MDR) organisms increasingly threatens global health and well-being. Plant natural products have been known for centuries as alternative medicines that can possess pharmacological characteristics, including antimicrobial activities. The antimicrobial activities of essential oil (Calli oil) extracted from the Calligonum comosum plant by hydro-steam di...

Rapidly growing embryogenic calli of different ages derived from the scutellum of mature seed embryos of indica rice (Rasi) and mature seeds of japonica rice (Taipei 309) was biolistically transformed. Plasmid pUCGUS containing the uidA gene encoding -glucuronidase was used to optimize transformation conditions using various combinations of helium pressure, target distance and gap distance. Pla...