To determine the role of the hormone prolactin and its receptor on the differentiation, growth, and metabolic activity of cells of bone marrow origin, prolactin receptor expression was assessed in bone marrow stromal cells. Using reverse transcription - polymerase chain reaction, BMS2 cells, a bone marrow stromal cell line, were shown to express prolactin receptors following adipocyte different...

A rat genomic DNA clone containing the 5’ flanking region, three exons, two introns, and a portion of a third intron of the rat prolactin gene was isolated and characterized. Sequence determinations were used to identify the exon-intron boundaries and analyze the DNA region upstream from the initiator methionine codon. Another genomic DNA clone that we previously characterized (Gubbins, E. J., ...

The results of the studies showed that by prolactin gene (PRL), highest yield was found in first-calf heifers with PRLBB genotype 4.7% -10.5% as compared to herd-mates other genotypes; and cows second third lactations – PRLAB 2.6-10.6%. In terms milk fat, animals had a higher rate than PRLAA genotypes 0.04-0.17 pp, especially lactation having most noticeable changes. By beta-lactoglobulin (BLG)...

Prolactin is best known for its effects of stimulating mammary gland development and lactogenesis. However, prolactin is a pleiotropic hormone that is able to affect several physiological functions, including fertility. Prolactin receptors (PRLRs) are widely expressed in several tissues, including several brain regions and reproductive tract organs. Upon activation, PRLRs may exert prolactin's ...

Mammary explants from pregnant ewes were cultured in the presence of insulin, cortisol and prolactin, either alone, or in combination. After 2 d of culture, total RNA was extracted from explants and the content of beta-lactoglobulin mRNA was estimated using a specific labelled cDNA probe. The mRNA for beta-lactoglobulin was only deinduced slowly in the absence of hormone during the culture. Pro...

The larynx of Xenopus laevis is a sexually differentiated vocal organ in which male muscle is entirely fast twitch and expresses high levels of a fast twitch myosin heavy chain gene, LM. Female muscle, however, is mostly slow twitch and expresses little LM. Androgen is unable to induce expression of LM until after metamorphosis is complete. The expression of LM during metamorphic and early post...

BACKGROUND Primary hyperparathyroidism (PHPT) is an endocrine disorder most commonly affecting women, suggesting a role for female hormones and/or their receptors in parathyroid adenomas. We here investigated the prolactin receptor (PRLr) which is associated with tumours of the breast and other organs. METHODOLOGY/PRINCIPAL FINDINGS PRLr expression was investigated in a panel of 37 patients w...

The recent report, “Serum prolactin level in psoriasis”, is very interesting 1. Ghiasi et al. concluded that “it seems that while intracutaneous prolactin may contribute to the pathogenesis of psoriasis, the probable role of pituitary-produced circulating prolactin in the disease process needs further investigation .” There are some concerns on this report. First, although Ghiasi et al. have st...