Zein can be extracted from maize (Zea mays) using ethanol solutions, and recovered from the extract by diluting with water. Dilution to 40% ethanol will precipitate a mixture of zein and maize lipid with 15% lipid. For possible zein applications, such as film production, purer zein is required. Most of the lipid can be removed from the precipitate by selective re-extraction with hexane or ethan...