نتایج جستجو برای: pqe

تعداد نتایج: 85  

Journal: :iranian biomedical journal 0
بیتا بخشی bita bakhshi مینا بوستان شناس mina boustanshenas مسعود قربانی masoud ghorbani

background: cholera toxin b subunit (ctb) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. the aim of this study was to compare the expression of native and mutant recombinant ctb (rctb) in pqe vector. methods: ctxb fragment from vibrio cholerae o1 atcc14035 containing the substitution of mutant ctxb for ami...

Journal: :iranian journal of biotechnology 2015
seyyedeh masumeh mirnurollahi azam bolhassani shiva irani noushin davoudi

background: hepatitis c virus (hcv) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (hcc). vaccination based on hcv capsid proteins has attracted a special interest for prevention of viral infections. the core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...

Journal: :Botanica Serbica 2022

The RUS (ROOT UVB SENSITIVE 1) proteins characterized by their unique DUF647 domain are widely distributed in eukaryotes. Their functional roles largely unknown except for the possible involvement of Arabidopsis RUS1 and RUS2 early seedling development. To investigate biochemical proteins, full length truncated were seamlessly fused with GFP cloned into prokaryotic expression vector pQE-100 whi...

Journal: :medical journal of islamic republic of iran 0
m azarnoosh from the department of biotechnology, pasteur institute of iran, tehran, islamic republic of iran. s zeinali sm tabatabaei a ziaee

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

ژورنال: :فصلنامه دامپزشکی و آزمایشگاه 0
نسترن سادات صدر شیرازی1، پرویز شایان1،2، الهه ابراهیم زاده1، بریگیته اکرت2

مقدمه و هدف: تشخیص متداول دو بیماری تیلریوز و بابزیوز در ایران بر اساس رنگ آمیزی گسترش ها ی خونی دامهای مشکوک با رنگ گیمسا می باشد. این نوع رنگ آمیزی بعنوان یک روش ساده ، ارزان و قابل استفاده در تمام آزمایشگاهها مطرح می باشد. اما از آنجایی که این رنگ آمیزی غیر اختصاصی است لذا تشخیص و تفکیک جنس و گونه اجرام انگلی بسیار مشکل و نیاز مند تجربه تخصصی بالا می باشد. برای مثال در بعضی موارد بعلت تشابه ...

Journal: :Proceedings of the VLDB Endowment 2022

The question of answering queries over ML predictions has been gaining attention in the database community. This is challenging because finding high quality answers by invoking an oracle such as a human expert or expensive deep neural network model on every single item DB and then applying query, can be prohibitive. We develop novel unified framework for approximate query leveraging proxy to mi...

2013
Hanumanthu Prasanna Lakshmi Sthanikam Yeswanth Uppu Venkateswara Prasad Dudipeta Vasu Vimjam Swarupa Pasupuleti Santhosh Kumar Mangamoori Lakshmi Narasu Potukuchi Venkata Gurunadha Krishna Sarma

Glucose-6-phosphate (G-6-P) formation in Staphylococcus aureus is catalysed by glucokinase (glkA) gene under high glucose concentration leading to upregulation of various pathogenic factors; therefore the present study is aimed in the cloning and characterization of glk A gene from S. aureus ATCC12600. The glk A gene was cloned in the Sma I site of pQE 30, sequenced (Accession number: JN645812)...

2013
Xianting Wu Jiajie Wu Yangfan Luo Jennifer Bragg Olin Anderson John Vogel Yong Q. Gu

Caffeic acid o-methyltransferase (COMT) is one of the important enzymes controlling lignin monomer production in plant cell wall synthesis. Analysis of the genome sequence of the new grass model Brachypodium distachyon identified four COMT gene homologs, designated as BdCOMT1, BdCOMT2, BdCOMT3, and BdCOMT4. Phylogenetic analysis suggested that they belong to the COMT gene family, whereas synten...

2012
N Sadr-Shirazi P Shayan B Eckert E Ebrahimzadeh N Amininia

BACKGROUND Because of the strong immunologic responses of surface protein TaSp in Theileria annulata infected host, we tried to characterize this protein in a T. annulata isolate from Iran. METHODS The RNA prepared from T. annulata infected cells was used to produce SMART-DS-cDNA. The Double strand cDNA was then amplified with primers derived from TaSp mRNA sequences. The PCR product was clon...

2011
W. P. Schleich

Wave-particle dualism is at the very heart of quantum mechanics. It manifests itself mostprominently in the double-slit experiment that has given rise to a fascinating discussion of “which-slit” information versus interference. In the present talk we analyze [1,2] two recent experiments[3,4] reporting simultaneous observation of “which-slit” and interference. Moreover, we introd...

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