Porous collagen scaffold is integrated with surface activated PLLA nanoparticles fabricated by lyophilizing and crosslinking via EDC treatment. In order to prepare surface-modified PLLA nanoparticles, PLLA was firstly grafted with poly (acrylic acid) (PAA) through surface-initiated polymerization of acrylic acid. Nanoparticles of average diameter 316 nm and zeta potential -39.88 mV were obtaine...

A contemporary procedure to grow artificial tissue is to seed cells onto a porous biomaterial scaffold and culture it within a perfusion bioreactor to facilitate the transport of nutrients to growing cells. Typical models of cell growth for tissue engineering applications make use of spatially homogeneous or spatially continuous equations to model cell growth, flow of culture medium, nutrient t...

Magnesium has been recently recognized as a biodegradation metal for bone substitute application. In the present work, porous magnesium-zinc scaffolds were prepared by powder metallurgical process and nano hydroxyapatite (HAP) coating on the Mg-3Zn (wt.%) scaffold was prepared by pulse electrodeposition and alkali treatment processes to improve the corrosion resistance of scaffold. The results ...

Scaffold design remains a main challenge in tissue engineering due to the large number of requirements that need to be met in order to create functional tissues in vivo. Computer simulations of tissue differentiation within scaffolds could serve as a powerful tool in elucidating the design requirements for scaffolds in tissue engineering. In this study, a lattice-based model of a 3D porous scaf...

B ioreactors allowing direct perfusion of culture medium through tissue-engineered constructs may overcome diffusion limitations associated with static culturing, and may provide flow-mediated mechanical stimuli. The hydrodynamic stress imposed on cells in these systems will depend not only on the culture medium flow rate but also on the scaffold three dimensional (3D) micro-architecture. We pe...

Bioreactors allowing direct perfusion of culture medium through tissueengineered constructs may overcome diffusion limitations associated with static culturing, and may provide flow-mediated mechanical stimuli. The hydrodynamic stress imposed on cells in these systems will depend not only on the culture medium flow rate but also on the scaffold three-dimensional (3D) micro-architecture. We perf...

Bioactive glass was supposed as a biodegradable material and designed as a scaffold to be used for bone reconstruction or regeneration. Bioactive glass scaffold with pore sizes ranged in 100-400μm in diameter was fabricated by sol-gel method, and the biocompatibility evaluation of bioactive glass scaffolds was also performed by culture in vitro models. Cells cultured in the extracts of bioactiv...

Skin wound healing is an important lifesaving issue for massive lesions. A novel porous scaffold with collagen, hyaluronic acid and gelatin was developed for skin wound repair. The swelling ratio of this developed scaffold was assayed by water absorption capacity and showed a value of over 20 g water/g dried scaffold. The scaffold was then degraded in time- and dose-dependent manners by three e...

At present there is no satisfactory treatment for deep osteochondral defects. Here we report the development of a biologic prosthetic composite containing periosteum from 2-month-old rabbits and a porous tantalum scaffold. When cultured under chondrogenic conditions, the composites form a robust hyaline-like cartilage outgrowth that is attached to the porous scaffold by fibrous tissue ingrowth....