Coffee plays an important role in the global economy, human health and society. It is one of major cash crops constituting backbone Nigerian economy before emergence re-emergence petroleum oil. Molecular markers are reliable valuable tool for analyzing polymorphism, screening selection desirable genotypes breeding programs. The study aimed at determining genetic diversity among coffee genotypes...

Montipora digitata is a species belonging to the Acroporidae. In Indo-Pacific region, M. widely distributed and dominant of scleractinian coral in South China Sea, however, there are currently no molecular markers suitable for assessing genetic diversity. Here, restriction-site associated DNA sequencing (RAD-seq) was used isolate characterize polymorphic microsatellite loci. A total 317,361 RAD...

In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (PIC) varied from 0.66 (Xgwm429) to 0.94 (Xgwm212 and Xgw...

Polyploidy plays an important role in the evolution of plant genomes. To enable the investigation of the polyploidy events within the genus Anemone, we developed eighteen microsatellite markers from the hexaploid species A. amurensis (Ranunculaceae), and tested their transferability in five closely related species. The number of total alleles (N(A)) for each resulting locus varied from one to e...

Fagopyrum represents an economically and nutritionally important crop which belongs to the group of pseudocereals. Buckwheat is referred be a functional with wide range use around world. Grains buckwheat are significant. They characterised by high content starch, proteins, flavonoids fibre. SCoT technique used for detection plant genes polymorphism, evaluation genetic diversity mapping regions ...

Ten Synthetic Hexaploid Wheats (SHWs) (2n = 6x = 42, AABBDD) were analyzed using 7 Randomly Amplified Polymorphic DNA (RAPD) primers to investigate the potential genetic diversity resided within the D-genome of common wheat. Total 190 fragments were amplified in which 141 were polymorphic showing 74 % of polymorphism. Polymorphism Information Content (PIC), Genetic Similarity (GS) Coefficient, ...

An assessment was made of the genetic variation of the Pulawska pig through the determination of polymorphism of 6 genes and 14 microsatellite sequences. The examinations covered 52 gilts included in a preservation breeding project. The identification of the alleles at microsatellite loci was performed in an ABI PRISM 310 GENETIC ANALYZER. Gene polymorphism was established by the PCR-RLFP metho...

In this study, Simple Sequence Repeat markers (SSR) were used to investigate the genetic variation between 49 cumin ecotypes collected from 9 different provinces of Iran. SSR primers Elap1479, Elap040 and Elap1493 showed the highest (89%), while Elap1340 and Elap017 showed the lowest (56%) number of polymorphic bands. Polymorphism information content (PIC) values varied between 0.18 - 0.37. The...

Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and palaestina Benth (Palestinian species using touch-up directed amplification of minisatellite region DNA (TU-DAMD) has been performed two separated sets; the first set (set A) initial annealing temperature was increased from 50 °C to 55 °C, whereas, second one B), it 60 by 0.5 °C/cycle during 10 PCR cycles. Fifteen DAMD primer...

in this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (ssr) alleles, covering all three wheat genomes. a total of 1557 allelic variants were detected for 126 ssr loci. the number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (pic) varied from 0.66 (xgwm429) to 0.94 (xgwm212 and xgwm515). the high­est po...