• A new in-column methacrylate-based polymerization procedure was developed for imaging the internal morphology of bare silica HPLC columns Polymerization performed in a commercial and home-made column The obtained silica/polymeric rod can be mechanically cut allowing cross section SEM allows mapping local external porosity packed could assistance to steer optimization packing processes columns...

The evaluation of weight average molecular weight (Mw) and molecular weight distribution represents one of the most controversial aspects concerning the characterization of low molecular weight heparins (LMWHs). As the most commonly used method for the measurement of such parameters is high performance size exclusion chromatography (HP-SEC), the soundness of results mainly depends on the approp...

Objective: The present study was aimed to develop a high performance liquid chromatographic method for simultaneous estimation of curcumin and quercetin and to implement the developed method for the estimation of curcumin and quercetin encapsulated in polymeric nanoparticles. Methods: Method development was carried out with different column, flow rate, elution mode, and buffer-solvent ratio to ...

The influence of solution chemistry and cell concentration on bacterial transport has been examined using Salmonella pullorum SA1685 and Escherichia coli O157:H7. A column was employed to determine the transport behavior and deposition kinetics with aquifer sand over a range of ionic strengths and cell concentrations. O157:H7 was found to be more adhesive than SA1685, with calculated deposition...

A novel polymeric monolith with hydrophilic interaction and strong anion-exchange mixed-mode has been fabricated for pressurized capillary electrochromatography by an in situ copolymerization of vinylbenzyl trimethylammonium chloride (VBTA) and bisphenol A glycerolate dimethacrylate (BisGMA). The optimization of the polymerization mixture composition has been investigated, and column characteri...

Quantification of the new immunosuppressant sirolimus (syn. rapamycin; Rapamune) in whole blood by chromatography is essential for its clinical use since no immunoassay is available although monitoring is mandatory. Here we report on a rapid and convenient liquid chromatography (LC-tandem mass spectrometry method and describe our practical experience with its routine use. Whole blood samples we...

BACKGROUND Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It has been speculated that lipid binds to the column material or the entire lipos...

In a liquid-liquid interface bioreactor, fungal cells locate in a hydrophilic polyacrylonitrile microsphere layer on an aqueous-organic interface. In this article, effects of hydrophobicity of the interface on n-decane hydroxylation activity of Monilliera sp. NAP 00702 was examined. (-)-4-Decanol production was significantly enhanced to 132% by addition of polytetrafluoroethylene.

A heterogeneous flow Suzuki–Miyaura coupling has been carried out. water or aqueous organic solution of aryl halides and arylboronic acids (left-hand side) was continuously injected into a column cartridge filled with convoluted polymeric Pd catalysts stabilizing auxiliary materials (orange balls) along sea sand (white balls). The products, which included liquid crystals, pharmaceutical compoun...