This work investigated polyethyleneimine (PEI)-introduced chitosan (CS) (CS/PEI) nanoparticles as non-viral carrier of plasmid DNA for rat mesenchymal stem cells (MSCs). The CS/PEI nanoparticles were prepared by the emulsifier-free emulsion polymerization of methyl methacrylate monomer induced by a small amount of t-butyl hydroperxide in the presence of different concentrations of PEI mixed wit...

We focused our study on the olfactory cells growth on biocompatible polymer films electrodeposited on a silicon microsystem. Several substrates such as polyethyleneimine (PEI), polypropyleneimine (PPI), and polypyrrole (PPy), acting as potentially good candidates for cell culture, were tested in order to allow cells to adhere and proliferate. During their growth, the evolution of their morpholo...

Polyethyleneimine (PEI) is an organic polycation used extensively as a gene and DNA vaccine delivery reagent. Although the DNA targeting activity of PEI is well documented, its immune activating activity is not. We recently reported that PEI has robust mucosal adjuvanticity when administered intranasally with glycoprotein antigens. Here, we show that PEI has strong immune activating activity af...

Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this paper, polyethyleneimine-capped silver nanoclusters (PEI-AgNCs) were prepared for the purpose of microRNA (miRNA) delivery. The resultant PEI-AgNCs were characterized by a photoluminescence assay and transmission electron microscopy. A cytotoxicity assay showed that PE...

Several nanocomposites based on Mesoporous Silica Nanoparticles (MSN), aluminum ions, and Polyethyleneimine (PEI) were synthesized by the combination of sol-gel post-synthesis methods. These mainly composed MSN with leading Al PEI species alone or in combination. The Al-MSN, MSN-PEI, Al-MSN/PEI samples characterized using FT-IR, XRD, SEM, EDX, zeta potential, nitrogen physisorption techniques. ...

There is a general necessity to improve the specificity and efficiency of the polymerase chain reaction (PCR), and exploring the PCR-enhancing mechanism still remains a great challenge. In this paper we report the use of branched polyethyleneimine (PEI)-based derivatives and hybrid nanocomposites as a novel class of enhancers to improve the specificity and efficiency of a nonspecific PCR system...

Ni-Co-SiC composite coatings were prepared by modified watt-type plating solution with 40 nm SiC particles. The effects of polyethyleneimine (PEI) on the dispersion nanoparticles and hardness corrosion resistance studied. electrode reaction process was measured cyclic voltammetry. results showed that when PEI concentration 0.06 g/L, peak current highest. distribution in relatively uniform coati...

A novel target specific small interfering RNA (siRNA) delivery system was successfully developed using polyethyleneimine (PEI)-hyaluronic acid (HA) conjugate. Anti-PGL3-Luc siRNA was used as a model system suppressing the PGL3-Luc gene expression. The siRNA/PEI-HA complex with an average size of ca. 21 nm appeared to be formed by electrostatic interaction between the negatively charged siRNA an...

Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene transfection. Graphene oxide (GO) is bound with cationic polymers, polyethyleneimine (PEI) with two different molecular weights at 1.2 kDa and...

BACKGROUND Radioimmunotherapy utilize a targeting antibody coupled to a therapeutic isotope to target and treat a tumor or disease. In this study we examine the synthesis and cell binding of a polymer scaffold containing a radiotherapeutic isotope and a targeting antibody. METHODS The multistep synthesis of a fluorescent or 149Promethium-labeled Trastuzumab-polyethyleneimine (PEI), Trastuzuma...