Objective: To explore a fast and sensitive method for screening T cell receptor (TCR) biases through comparison of silver staining for polyacrylamide gel electrophoresis and ethidium bromide staining for agarose gel electrophoresis. Methods: The lymphocytes were isolated from peripheral blood which collected from ten patients with T1DM; the total RNA was extracted followed by transformed to cDN...

Preparative polyacrylamide gel electrophoresis has been used in the final stages of purification to prepare horse serum cholinesterase which was at least 9.5% pure, as judged by analytical polyacrylamide gel electrophoresis. The starting material, already purified about 109-fold by the Strelitz method, was purified an additional 89-fold by the procedure. The method involved Sephadex G-ZOO and S...

Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN) have been compiled and characterized. Usefulness and applicability of the systems for clinical investigations are discussed and illustrated by...

Genetic diversity in 30 genotypes of Pumpkins was collected from unexplored mountainous areas Khyber Pakhtunkhwa, Pakistan investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total 35% genetic disagreement observed th...

Earthworms possess antioxidant, antibacterial, antitumor, and hemolytic properties. To recognize the molecules responsible for various biological activities of earthworm’s coelomic fluid, a detailed knowledge about its protein contents is required. The aim of this study was to characterize the proteins present within the coelomic fluid of Eisenia foetida earthworm. Polyacrylamide-gel-elec...