Background and Aims: World Health Organization Global Polio Laboratory Network (GPLN) plays a critical role in the Global Polio Eradication Initiative. Cell culture methods (mostly RD and L20B cell lines) have been used for Enteroviruses and polioviruses isolation, respectively. Cross-contamination among L20B and RD cell lines causes the problem in accuracy of poliovirus surveillance and decrea...

Non-polio enteroviruses (NPEVs) are primary causative agents of aseptic meningitis, encephalitis, hand, foot and mouth disease (HFMD), myocarditis in infants children immunocompromised individuals. However, there no approved treatments for NPEVs. It has been reported that flavonoids abundantly found plants have antiviral activities. In this study, we explored the potential quercetin-3-glucoside...

BackgroundAcute Flaccid Paralyses Surveillance (AFPS) monitors the emergence of polioviruses and can track Non-Polio Enteroviruses (NPEVs). We report AFPS activity in Lombardy region (Northern Italy) from 2016 to 2018.MethodsFecal respiratory samples were collected children <15 years who met WHO definition an AFP case, analyzed by virus isolation cell cultures (RD/L20B) a one-step real-time RT-...

Non-polio enteroviruses (NPEVs) have been reported frequently in association with acute flaccid paralysis (AFP) cases during Polio Surveillance Programs (PSPs) worldwide. However, there is limited understanding on the attributes of their infections. This study reports characteristics of NPEVs isolated from AFP cases, investigated during PSPs held in 2009-2010, in Karnataka and Kerala states of ...

Enteroviruses (EVs) cause severe outbreaks of respiratory and neurological disease as illustrated by EV-D68 and EV-A71 outbreaks, respectively. We have mapped European laboratory capacity for identification and characterisation of non-polio EVs to improve preparedness to respond to (re)-emerging EVs linked to severe disease. An online questionnaire on non-polio EV surveillance and laboratory de...