The cross-resistances of several mutants of Chinese hamster ovary cells which have been obtained after one and two selection steps in the presence of the microtubule inhibitor podophyllotoxin ( P o d R~ and P o d R" mutants, respectively) towards various other inhibitors of microtubule assembly (e .g . , colchicine, Colcemid, vinblastine, griseofulvin, maytansine, steganacin, nocodazole, and ta...

The cross-resistances of several mutants of Chinese hamster ovary cells which have been obtained after one and two selection steps in the presence of the microtubule inhibitor podophyllotoxin ( P o d R~ and P o d R" mutants, respectively) towards various other inhibitors of microtubule assembly (e .g . , colchicine, Colcemid, vinblastine, griseofulvin, maytansine, steganacin, nocodazole, and ta...

Podophyllotoxins represent an important and unique position among natural products. They serve as the starting material for the semi-synthetic anticancer drugs etopside and teniposide, so considerable attention has focused on the availability of these aryltetralin lignans. Linum is one of the genera, with some reports on the presence of podophyllotoxin and related compounds, such as L. album, L...

Eastern red cedar (Juniperus virginiana L. Cupressaceae) occurs naturally throughout eastern North America and has been identified as a source of podophyllotoxin. Podophyllotoxin is used to manufacture drugs for treatment of cancer, rheumatoid arthritis, genital warts, psoriasis, and multiple sclerosis. Leaf material was collected from Juniperus plants located in northern Mississippi once a mon...

Three types, esters, amides and 1,2,3-triazoles, of ferrocenyl-podophyllotoxin conjugates were synthesised, and their anticancer activity was evaluated. We observed that the most potent ferrocenyl derivatives were esters. Esters 15, 16 and 17 acted in a similar way to podophyllotoxin, i.e. reduced the number of G1 phase cells and induced G2/M blockage, while esters 14 and 18 and amide 19 blocke...

Podophyllum species, the source of important secondary metabolite, podophyllotoxin, is over-exploited for production anti-cancer drugs endangering this genus globally. Lack complete knowledge on podophyllotoxin biosynthesis a major drawback in its cultivation and identification alternative plants. The current study secoisolariciresinol dehydrogenase, dirigent protein oxidase pluviatolide syntha...

Pharmacological evidence is presented for the involvement of microtubules in the process of fast axoplasmic transport. A quantitative measure of the inhibition of axoplasmic transport in an in vitro preparation of rat sciatic nerve is described. The alkaloids colchicine, podophyllotoxin, and vinblastine, which are known both to disrupt microtubules and to bind to the protein subunit of microtub...

the natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. in order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect...

INTRODUCTION Topical application of podophyllin is a routine procedure in patients with ano-genital warts. Podophyllin is a crude plant extract and is therefore not a well-defined product. It may contain variable amounts of the active lignan podophyllotoxin and the majority of the dry weight of podophyllin is made up of substances never identified. OBJECTIVE The purpose of the study was to es...

Time-resolved laser flash photolysis and pulse radiolysis have been used to study the chemical activity of podophyllotoxin(PPT) and etoposide(VP-16). The mechanism of photoionization of etoposide and podophyllotoxin has been confirmed and illustrated. It is demonstrated that VP-16 and PPT in aqueous solution can be photoionized at 248 nm to give hydrated electron and neutral radical resulting f...