نتایج جستجو برای: plasmid by the restriction enzymes then
تعداد نتایج: 17032277 فیلتر نتایج به سال:
Background and Objective: Toxoplasmosis, caused by an intracellular protozoan parasite, Toxoplasma gondii, is widespread throughout the world. Being a cause of congenital disease and abortion in humans and in domestic animals, the disease is of major medical and veterinary importance. In addition, it has increased importance due to toxoplasma encephalitis in AIDS patients. Therefore, developi...
Aspartic proteases are a relatively small group of enzymes which express in various nematodes including Onchocerca volvulus. An estimation of the gene copy number corresponding to the OV7A clone, which contains a cDNA insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of O. volvulus, was made by slot blot analysis in a closely related species O. gibsonig...
iv abstract this study examined the linguistic behaviors of two iranian efl teachers each of them teaching learners of two similar proficiency levels, a beginner level and an intermediate level, to investigate the relationship between the learners proficiency levels and the amounts and purposes for l1 use by the two teachers. the study was carried out to investigate whether there were differe...
Two plasmid-carried restriction-modification (R-M) systems, EcoRI (from pMB1 of Escherichia coli) and Bsp6I (from pXH13 of Bacillus sp. strain RFL6), enhance plasmid segregational stability in E. coli and Bacillus subtilis, respectively. Inactivation of the endonuclease or the presence of the methylase in trans abolish the stabilizing activity of the R-M systems. We propose that R-M systems med...
phatic communion is a cultural concept which differs across cultures. according to hofstede (2001), the u.s. tends to have individualistic culture; however, asian countries tend to have collectivistic cultures. these cultures view phatic communion differently. in individualistic cultures like u.s., phatic communion reflects speakers’ socio-cultural relationships in conversations. to see whether...
The present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza A M2-protein (M2e), which was fused to the N-terminus of the truncated HSP70 (HSP70359–610) molecule as a new approach for future vaccine research against influenza A. The amplified fragments, M2e and HSP70359-610 genes, were gel-purified. The products were then single digested with Bam...
abstract objectives gradual increase length and complexity of utterance (gilcu) therapy method is a form of operant conditioning. this type of treatment is very precise and controlled that is done in 54 steps in 3 speech situations consisted of monologue, reading and conversation. this study aimed to examine the effects of gilcu treatment method on reduction of speech dysfluency of school-age...
Introduction: Helicobacter pylori (H. pylori) infection has remained as a global health problem. Animal studies demonstrated the role of H. pylori oipA gene in the development of gastric cancer. The aim of this study was the cloning and expression of Helicobacter pylori oipA gene in a bicistronic vector harboring mice IL-18 gene. Materials and methods: The target gene encoding oipA was amp...
background brucellosis is an important cosmopolitan infection disease caused by organisms belonging to the genus brucella. the cgt gene (cyclic β-1, 2 glucan transporter gene) is a virulent factor in brucella genus. the present study was conducted with the aim of cloning and expression of brucella cgt gene. materials and methods brucella melitensis cgt gene was amplified from extracted chromoso...
in chapter 1, charactrizations of fragmentability, which are obtained by namioka (37), ribarska (45) and kenderov-moors (32), are given. also the connection between fragmentability and its variants and other topics in banach spaces such as analytic space, the radone-nikodym property, differentiability of convex functions, kadec renorming are discussed. in chapter 2, we use game characterization...
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